<正>研究了里氏木霉以纸浆为碳源分批补料制备纤维素酶。里氏木霉在10L发酵罐中以纸浆为碳源间歇式发酵合成纤维素酶,培养基中碳源浓度为15g/L时,滤纸酶活力、纤维二糖酶活力、酶产率和酶得率分别为2.15FPIU/mL、0.20IU/mL、16.3FPIU/(L·h)和143.3FPIU/g;碳源浓度提高到27.5g/L,采用分批补加碳源的方法,滤纸酶活力、纤维二糖酶活力、酶产率和每克纸浆酶得率分别为3.90FPIU/mL、0.35IU/mL、23.2FPIU/(L·h)和141.8FPIU。研究表明,提高培养基中碳源浓度,采用补料分批发酵技术,产酶时酶活力和酶产率随着培养基中碳源浓度的提高而提高,且保持酶得率不变,达到了降低产酶成本的目的。

Batch cellulase production with 15 g/L sulfite pulp in 10 L fermenter by Trichoderma reesei,the filter paper activity,cellobiase activity,cellulase productivity and cellulase yield were 2.15 FPIU/mL,0.20 IU/mL,16.3 FPIU/(L·h) and 143.4 FPIU/g pulp,respectively;Raising substrate concentration from 15 g/L to 27.5 g/L with a fed-batch mode,the filter paper activity,cellobiase activity,cellulase productivity and cellulase yield were 3.90 FPIU/mL,0.35 IU/mL,23.2 FPIU/(L·h) and 141.8 FPIU/g pulp,respectively.In contrast to batch process,fed-batch process can increase cellulase activity and cellulase productivity,retain the cellulase yield,and reduce the cost of cellulase production.