Abstract: Urbanization has changed the landscape structure of urban and resulted in lots of natural phenomena and ecological processes worsen.This paper studied the dynamical change of landscape pattern of Nanjing city since 1985 by using GIS and major landscape index with Landsat TM imageries in 1985,1995 and landsat ETM~+ in 2000.The results showed that landscape structure of Nanjing changed dramatically in the course of rapid urbanization.The natural and semi-natural landscape such as cultivated land,greenbelt,water and unused land became man-made landscape.In general,landscape diversity and average area of the patches increased.Fragmentation F_i,separation N_i,and lacunarity A(r) decreased.The urban exploitation tends to regulation and rationalization.