Abstract: The modified GD medium was suitable to the development of embryos in vitro of Pinus elliottii. The tip sprout carrying cotyledons as explants was more easily to build the regeneration system for P. elliottii. Axillary buds were induced from modified GD medium supplemented with 6-BA (3 mg/L) and NAA (0.05 rag/L). The differentiation frequency was very high, reaching to 73. 3%. The half MS medium supplemented with NAA (0.05 rag/L) could greatly promote the root induction of the regenerated plantlets in P. elliottii, in which the rate of induction was 60%. The 1/2 modified GD medium without hormone had the advantage for the root elongation.