Abstract: In the experiment, six Chinese chestnut cultivars, including Yangshan oil chestnut, Tielitou chestnut, Double-season chestnut, Shao 18 chestnut and Heyuan oil chestnut were used as paternal cultivars for Nongda No. 1 chestnut. Yangshan oil chestnut had a common florescence time of 4 days with Nongda No. 1 chestnut, followed by Tielitou (3 days) and other chestnut varieties(2 days). Yangshan oil chestnut had the longest and heaviest male inflorescence, followed by Tielitou, Double-season chestnut and Nongda No. 1 chestnut in a decreasing sequence. Nongda No. 1 chestnut pollinated with Tielitou chestnut, DouBle-season chestnut and Yangshan oil chestnut had a fertile seed ratio of 91.25%, 80.00% and 76.14% respectively. When self pollinated and artificially pollinated with its own pollens, the ratios were 19.10% and 10.82%. respectively. Metaxenia phenomenon was observed in the single seed weight. Based on the experimental results, we believed that Tielitou, Shao 18 chestnut and Heyuan oil chestnut were suitable paternal cuttivars for Nongda No. 1 chestnut.