碳源对里氏木霉β-聚糖酶合成的影响

董博,连之娜,勇强,余世袁*

南京林业大学学报(自然科学版) ›› 2005, Vol. 29 ›› Issue (06) : 88-90.

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南京林业大学学报(自然科学版) ›› 2005, Vol. 29 ›› Issue (06) : 88-90. DOI: 10.3969/j.jssn.1000-2006.2005.06.022
研究论文

碳源对里氏木霉β-聚糖酶合成的影响

  • 董博,连之娜,勇强,余世袁*
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Effects of Carbon Sources on the Biosynthesis of β-glycanases by Trichoderma reesei

  • DONG Bo, LIAN Zhi-na, YONG Qiang, YU Shi-yuan*
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摘要

<正>对大戟科4种药用植物大戟、泽漆、乌桕、重阳木内生真菌进行分离鉴定,并检测其抑菌活性。结果表明:4种植物均含有内生菌,乌桕中获得13株内生菌,鉴定12株,分别属于丝核菌属、小菌核菌属、拟盘多毛孢属、小单头孢属、毛壳菌属、壳小圆孢属、棒盘孢属、交链孢属;京大戟中获得9株内生菌,鉴定8株,分别属于镰刀菌和交链孢属;泽漆中获得6株内生菌,鉴定4株,属于交链孢属;重阳木中获得15株内生菌,鉴定了4株,分别属于小穴壳属、壳小圆孢属、拟茎点霉属。茎中均有内生菌,种子和重阳木叶中均未分离到。总计43株内生菌中有11株有稳定的抑菌活性,占总菌株的25.58%,有12株有不稳定的抑菌活性,占27.91%。用绿豆发芽试验检验表明,仅有1株菌在绿豆幼苗上形成病斑,其余均生长良好,且21株表现出对绿豆芽的促进作用。

Abstract

The effects of carbon sources on the preparation of β-glycanases were investigated. The result showed: The filter paper activity,CMCase activity, cellobiase activity and xylanase activity were 1.03 FPIU/mL, 0.54 IU/mL, 0.08 IU/mL and 149.7 IU/mL, respectively, when 15 g/L corncob employed as carbon source by Trichoderma reesei. The poor-xylanase cellulase was synthesized under 15 g/L kraft pulp as carbon source. The poor-cellulase xylanase, which was suitable for biobleaching,was formatted when the T. reesei cultivated in the same concentration of unpurified xylan,and the ratio of xylanase to CMCase was 785.4.

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董博,连之娜,勇强,余世袁*. 碳源对里氏木霉β-聚糖酶合成的影响[J]. 南京林业大学学报(自然科学版). 2005, 29(06): 88-90 https://doi.org/10.3969/j.jssn.1000-2006.2005.06.022
DONG Bo, LIAN Zhi-na, YONG Qiang, YU Shi-yuan*. Effects of Carbon Sources on the Biosynthesis of β-glycanases by Trichoderma reesei[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2005, 29(06): 88-90 https://doi.org/10.3969/j.jssn.1000-2006.2005.06.022
中图分类号: Q556   

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