Abstract: This paper presents an unreported overall study about using bulb scale as the initial explant and MS as the basic medium with different hormones in different concentration levels to induce Lilium bulbiferum cv. ’Marco polo’ differentiating little bulbs so as to to find the best technical line to realize lilly factory production. The result reveals that "Marco polo" differentiates into bulbous projections and forms shoots directly from explants. Using interior whole scale of a bulb as the explant with the scale back lying on the medium is the best material to induce "Marco polo" to differentiate little bulbs, and with that on the medium of MS+ 6 -BA (1.0mg/L)+IBA (0.3mg/L), the average inducing coefficient can reach 2. 52. The best reproduction medium for "Marco polo" is MS+6 -BA (0.3mg/L)+IBA (0.3mg/L), on which reproducing coefficient can reach 2.60.1/2MS + 6 - BA (0.3mg/L) + IBA (0.7mg/L) is the best medium for "Marco polo" to root, and its rooting rate can be 100%. The material for transplanting is the combination of river mud and pearly rock (cubic comparison is 7: 3). The survival rate of Lilium shoots can reach 98%.