银杏叶片蛋白质含量动态变化的电泳分析

王改萍,彭方仁*,李生平

南京林业大学学报(自然科学版) ›› 2006, Vol. 30 ›› Issue (04) : 114-118.

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南京林业大学学报(自然科学版) ›› 2006, Vol. 30 ›› Issue (04) : 114-118. DOI: 10.3969/j.jssn.1000-2006.2006.04.027
研究论文

银杏叶片蛋白质含量动态变化的电泳分析

  • 王改萍,彭方仁*,李生平
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Studies on Changes of Proteins in the Leaves of Ginkgo biloba by SDS-PAGE and IFE-SDS-PAGE

  • WANG Gai-ping, PENG Fang-ren*, LI Sheng-ping
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摘要

<正>以完全随机试验设计对不同土壤水分条件下浙江楠幼苗生长及某些生理特性进行了研究。结果表明:随着土壤含水率的增加,单叶面积、单叶鲜重、单叶干重、比叶面积逐渐增大。但叶片厚度、肉质度减小;叶片的叶绿素含量对土壤水分状况较为敏感,5种水分处理下苗木叶片的叶绿素舍量有较大的差异。土壤干旱和水涝均影响叶绿素a和叶绿素b的合成。在轻度干旱时。苗木的含水率、自然饱和亏缺和水势的变化不大。-而在重度干旱时,苗木的舍水m降低。自然饱和亏缺增大。水势降低。叶片的光台能力降低,苗木生长缓慢,生物量下降,说明土壤水分状况对净光合作用速率有重要影响。

Abstract

The techniques of SDS-PAGE, IFE-SDS-PAGE, and Brilliant Blue and CommassieDye were used to study the fractions of Vegetative Storage Proteins (VSPs) and the concentrationof the soluble protein in the leaves of Ginkgo biloba. The results showed that the concentrationof the soluble protein in the leaves of Ginkgo biloba (both from a male tree and afemale tree) was higher during the growing period in one year. But it began to decrease at thelast ten-day of October and decreased to the lowest level at the middle ten-day of November, reflecting nitrogen nutrient transfer from the leaves to the other tissues of a tree when theleaves began to fall down. Based on the SDS-PAGE bands, it was found that VSP 55 kD bandstill could be clearly observed in the lane of the middle of October. While this VSP band wasundetectable in the lane of the beginning of November for a female tree, and in the middle ofNovember for a male tree. The ease of VSP 32kD was very similar to VSP 55kD. Only differencewas that VSP 32kD could not be detected until the beginning of November for a femaletree or for a male tree. With the technique of IFE-SDS-PAGE, the seasonal variations ofVSPS 55kD and 32kD were confirmed, and also it was found that another VSP, 18kD, sharplydecreased in the middle of November for a female tree and decreased in the beginning ofNovember for a male tree.

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王改萍,彭方仁*,李生平. 银杏叶片蛋白质含量动态变化的电泳分析[J]. 南京林业大学学报(自然科学版). 2006, 30(04): 114-118 https://doi.org/10.3969/j.jssn.1000-2006.2006.04.027
WANG Gai-ping, PENG Fang-ren*, LI Sheng-ping. Studies on Changes of Proteins in the Leaves of Ginkgo biloba by SDS-PAGE and IFE-SDS-PAGE[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2006, 30(04): 114-118 https://doi.org/10.3969/j.jssn.1000-2006.2006.04.027
中图分类号: S792.95    Q591   

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