南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (02): 9-12.doi: 10.3969/j.jssn.1000-2006.2007.02.003

• 研究论文 • 上一篇    下一篇

缺磷胁迫下马尾松和杉木苗根系有机酸的分泌

俞元春1,余健2,房莉2,姜德锋1   

  1. 1. 南京林业大学森林资源与环境学院, 江苏 南京 210037; 2. 安徽师范大学国土资源与旅游学院, 安徽 芜湖 241000
  • 出版日期:2007-04-18 发布日期:2007-04-18

Organic Acids Exudation from the Roots of Cunninghamia lanceolata and Pinus massoniana Seedlings Under Low Phosphorus Stress

YU Yuan-chun1, YU Jian2, FANG Li2, JIANG De-feng1   

  1. 1. College of Forest Resources and Environmenl Nanjing Forestry University, Nanjing 210037, China; 2. College of National Territorial Resources and Tourism Anhui Normal University, Wuhu 241000, China
  • Online:2007-04-18 Published:2007-04-18

摘要: <正>以壳聚糖微球为载体构建了固定化p葡萄糖苷酶,研究了固定化和游离β-葡萄禧苷酶的酶学性质。结果表明:固定化和游离β-葡萄糖苷酶的最适pH均为4.5;固定化β-葡萄糖苷酶的最适温度为60C。比游离酶纸5C;固定化p-葡萄糖苷酶的pH稳定性和贮存稳定性明显高于游离酶,但热稳定性略低干游离酶;固定化和游离p葡萄糖苷酶的表观米氏常数和米氏常数分别为0.52mmol/L和2.4mmol/L;固定化β-葡萄糖苷酶重复分批酶解10g/L的纤维二糖。其操作半衰期为31d左右。

Abstract: Organic acids exudation from the roots of Chinese fir (Cunninghamia Zanceolata) and Masson pine (Pinus massoniana) seedlings under low phosphorus stress was studied with solution culture method. The results revealed that organic acids exudation from the roots of Chinese fir and Masson pine seedlings under low phosphorus stress increased. Compared with P3(KH2PO4, 0.5 mmol/L), the average organic acids exudation from the root of Masson pine seedling under P, , (KH2PO4, 0 mmol/L), P~ (KH2PO4, 0.03 retool/L) and P2 (KH2PO4, 0.09 rnmol/L) increased 328.6~, 267.9~ and 126.40//00 respectively. Masson pine from Zhejiang province had the highest organic acids exudation. Under the low phosphorus stress, the kinds of increased organic acids exudation from the different provinces of Chinese fir and Masson pine were different. Masson pine from Zhejiang province mainly increased oxalic acid, tartaric acid, citric acid and malic acid exudation, from Guangxi Province mainly increased oxalic acid and tartaric acid exudation, from the Guizhou Province mainly increased oxalic acid, tartaric acid and malic acid exudation. Chinese fir mainly increased oxalic acid and tartaric acid exudation.

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