固定化β-葡萄糖苷酶的酶学性质

朱均均,江小华,勇强*,余世袁

南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (03) : 29-33.

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南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (03) : 29-33. DOI: 10.3969/j.jssn.1000-2006.2007.03.006
研究论文

固定化β-葡萄糖苷酶的酶学性质

  • 朱均均,江小华,勇强*,余世袁
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Enzymology Characteristics of Immobilized β-glucosidase

  • ZHU Jun-jun, JIANG Xiao-hua, YONG Qiang*, YU Shi-yuan
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摘要

<正>可生物分解的纳米复合材料是新一代材料,微米和纳米级纤维素纤丝具有环保性和潜在的低密度及高强度,可用来制造能生物分解的纳米复合材料。为了描述用一种新的机械方法从Lyocell纤维中分离出的纤丝的特性,以及探索这种纤丝能否增强生物分解的聚合物,笔者用偏振光显微镜(PLM)和扫描电子显微镜(SEM)研究了纤丝的几何特性,用宽角X光衍射法(WAXD)分析了其结晶度,纤维的纤丝化程度用水分保持值(WRV)进行了评价;纤丝增强的聚乳酸(PLA)纳米生物复合材料由模压法制备,用拉伸试验来检测其力学强度,采用SEM来观察其断面特性。结果表明:纤丝提高了PLA的弹性模量和拉伸强度,但纤丝和聚合物基质之间的结合强度很弱。

Abstract

The enzymology characteristics of immoobilized and free 13-glucosidase were investigated. The optimal pH of immobilized and free 13-glucosidase was 4.5. The optimal temperature of immobihzed β-glucosidase was 60 C, which was 5 C lower than that of free 13 glucosi dase. hs stabilities of pH and storing were obviously higher than that of free β-glucosidasc, but its thermal stability was a little lower than that of free 13-glucosidase. The apparent Km and Km of immobilized and free 13-glucosidase were 0.52 mmol/L and 2.4 mmol/L, respec tively. The immobilized 13 glucosidase was used to hydrolyze 10g/L cellobiose solmion re peatedly, its half life time was about 31days.

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朱均均,江小华,勇强*,余世袁. 固定化β-葡萄糖苷酶的酶学性质[J]. 南京林业大学学报(自然科学版). 2007, 31(03): 29-33 https://doi.org/10.3969/j.jssn.1000-2006.2007.03.006
ZHU Jun-jun, JIANG Xiao-hua, YONG Qiang*, YU Shi-yuan. Enzymology Characteristics of Immobilized β-glucosidase[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2007, 31(03): 29-33 https://doi.org/10.3969/j.jssn.1000-2006.2007.03.006
中图分类号: TQ920.1    Q814.2   

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