南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (05): 49-52.doi: 10.3969/j.jssn.1000-2006.2007.05.011

• 研究论文 • 上一篇    下一篇

兰科丝核菌类真菌对铁皮石斛生长的影响

亢志华,韩素芬,韩正敏*   

  1. 南京林业大学森林资源与环境学院, 江苏 南京 210037
  • 出版日期:2007-10-18 发布日期:2007-10-18

Effects of Orchidaceous rhizoctonias on the Growth of Dendrobium candidum

KANG Zhi-hua, HAN Su-fen, HAN Zheng-min*   

  1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China
  • Online:2007-10-18 Published:2007-10-18

摘要: <正>采用两级超滤、DEAE-Sepharose FF阴离子交换层析、CM-Sepharose FF阳离子交换层析和Sephadex G-100凝胶渗透层析等分级纯化步骤,从里氏木霉纤维素酶系中分离纯化得到电泳纯的3个内切葡聚糖酶组分EGⅠ、EGⅡ和EGⅢ,2个外切葡聚糖酶组分CBH Ⅰ和CBH Ⅱ和1个β-葡萄糖苷酶组分,它们对各自底物的比活力分别为176.35、153.96、64.22、16.86、4.82、31.00IU/mg,米氏常数分别为6.70、8.46、13.22、1.37、3.46、2.20mg/mL。同一类酶组分的米氏常数Km越大,转换数Kcat越小。分离纯化所得EG Ⅰ、EG Ⅱ、EG Ⅲ、CBH Ⅰ、CBH Ⅱ和GB等酶组分的分子量分别为50、46、25、65、58、75kDa。

Abstract: Three strains which belong to Orchidaceous rhizoctonias were inoculated into the tissue culture seedlings of Dendrobium candidum. They were GDB254, MLX102, CLN103 which belong to Epulorhiza sp. and Ceratorhiza sp. After 65 days, the average fresh weight of seedlings was higher than those of constrastive seedlings; the weight of seedlings, which were inoculated with the three strains were all extremely significant (α=0.01). Chloropphll a、 b content of seedlings were higher than that those of contrastive seedlings. Through observation on the microstructure of mycorrhiza. It was found that there were pelotons in cortical and vascular tissue. So it was confirmed that the relationship of symbiosis was already formed, the growth and chloropphll a、 b content of Dendrobium candidum were promoted by the mycorrhizal fungi. GDB254, MLX102, CLN103 were good mycorrhizal strains of D. candidum.

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