<正>采用不同浓度的H2O2和抗菌素处理松材线虫虫卵和松材线虫虫体，筛选松材线虫无菌化处理有效途径。多种方法的比较表明，采用高浓度抗菌素和低浓度H2O2交替并反复处理松材线虫虫体，获得无菌线虫的比例高，线虫生命力强。将获得的无菌松材线虫与带菌线虫分别用。PSA和玉米粒上培养的灰葡萄孢进行人工培养，发现线虫生长繁殖情况无明显差异。

To detect the effect of count data with many zeros on the zeroinflated poisson regression model, the authors studied the global diagnostics analysis and using the completedata log-likelihood function and the EM algorithm. The one-step approximation of parameter estimates and some statistics, such as generalized cook distance, Q-distance, are obtained. Meantime, the generalized leverage was discussed based on the EM algorithm. Finally, a numerical example was given to illustrate our results.