南京林业大学学报(自然科学版) ›› 2009, Vol. 33 ›› Issue (01): 0-.doi: 10.3969/j.jssn.1000-2006.2009.01.001
• 特稿 • 下一篇
李德伟,John C Sutton
出版日期:
2009-02-18
发布日期:
2009-02-18
基金资助:
LI Dewei,John C Sutton
Online:
2009-02-18
Published:
2009-02-18
摘要: 以仙客来叶圆片方法对灰霉菌Botrytis cinerea的生防效果进行了评估,结果发现Clonostachys rosea和Trichoderma koningii抑制B. cinerea的效果达94%~100%,其效果优于所测试的从仙客来上分离的真菌和细菌的多数菌株。用整株仙客来测试生防菌效果表明,C.rosea在叶片、花瓣、叶柄和花梗上抑制B.cinerea效果分别达96%、58%、53%、77%;而Trichoderma koningii的效果分别达100%、42%、100%、 90%。 以析因试验设计对B.cinerea和C.rosea或T.koningii施用浓度在生防效果上的交互效应进行了测定,结果表明:当C.rosea的接种浓度是病原菌浓度的10倍或10倍以上时,其在叶片、花瓣、叶柄和花梗上抑制B.cinerea的效果分别达73%~100%、52%~86%、100%、54%~68%;当T.koningii的接种浓度为106个/mL或以上时,其抑制B.cinerea在仙客来(接种浓度为103~106个/mL时)各部分发生的效果分别为:叶片100%、叶柄62%~100%、花瓣38%~96%、花梗 56%~100%。C.rosea在叶龄为2、3、5、6 周的叶片上的生防效果更好,而在叶龄为4 周的叶片上效果差些,在叶龄为1 周的叶片上无效果。T.koningii在叶龄为1~6 周的叶片上均可有效地抑制B.cinerea的发生,抑制效果达89%~100%。C.rosea和T.koningii均是在温室内防治仙客来灰霉菌B.cinerea的具潜力的有效生防菌,但T.koningii的防治效果显著地优于C.rosea的效果。
中图分类号:
李德伟,JohnCSutton. 拮抗微生物防治仙客来灰霉菌Botrytis cinerea的评估[J]. 南京林业大学学报(自然科学版), 2009, 33(01): 0-.
LI Dewei,John C Sutton. Evaluation of antagonistic microorganisms for biocontrol of Botrytis cinerea in cyclamen[J].Journal of Nanjing Forestry University (Natural Science Edition), 2009, 33(01): 0-.DOI: 10.3969/j.jssn.1000-2006.2009.01.001.
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