通过不同的脉冲磁场处理,观察了磁场对松褐天牛(Monochamus alternatus)幼虫存活和发育的影响,探讨了脉冲磁场处理下松褐天牛幼虫在活力、生长发育、细胞膜透性和纤维素酶活性的动态变化。结果表明：(1)脉冲磁场在一定程度上可杀死松褐天牛幼虫,脉冲前沿宽度是影响松褐天牛生存的主要因子,5ms(磁场峰值3180Gs)脉冲前沿宽度处理松褐天牛幼虫的平均校正死亡率为63％,脉冲前沿宽度越大,松褐天牛幼虫校正死亡率越高；(2)松褐天牛受到脉冲磁场作用后,幼虫出现畸形而不能正常化蛹，出现滞育现象,不能正常进入下一个生活周期。化蛹所需平均时间延长；(3)脉冲磁场可破坏松褐天牛幼虫的细胞膜结构,细胞内部分电解质外渗，脉冲前沿宽度是主要影响因子,脉冲前沿宽度越大,松褐天牛幼虫相对电导率越高；(4)脉冲磁场作用后,松褐天牛幼虫纤维素酶活性降低,脉冲前沿宽度越大,脉冲串个数越多,纤维素酶活性越低,C1酶降低程度最大。
Abstract
With different pulsed magnetic field processing, the effect of magnetic field on the survival and growth of Monochamus alternatus larva were observed. After the magnetic field processing, the dynamic change of the larva of M. alternatus with regard to the activity, growth, membrane permeability and cellulosic enzyme activity were discussed. The results showed that: (1)After treated with pulsed magnetic field, the larva of M. alternatus could be killed to some extent and pulse rise time was the main factor that influenced the survival of M. alternatus. When 5ms (magnetic peak 3180Gs) pulse rise time treated the M. alternatus larva, the average corrected mortality could reach to 63%. The greater the pulse rise time, the higher corrected mortality of M. alternatus larva would be. (2)When subjected to pulsed magnetic field, the larva of M. alternatus would become deformed such that it could not pupate as usual; it would suffer diapause phenomenon and could not enter next life cycle normally or the average time needed to pupate increased. (3)Pulsed magnetic field could destroy the cell membrane structure of the larva of M. alternatus, causing a part of electrolyte in the cell to leak out. The effect of the pulsed magnetic field on the membrane permeability of M. alternatus was assessed with relative conductivity and it was found that pulse rise time was the main impact factor, the greater the pulse rise time, the higher relative conductivity of M. alternatus larva would be. (4)After the treatment of pulsed magnetic field, the cellulosic enzyme activity of M. alternatus larva would be degraded and the greater the pulse rise time and the more the pulse train number, the lower the cellulosic enzyme activity would be, with C1 enzyme activity lowered most.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
［1］张星耀,骆有庆.中国森林重大生物灾害［M］.北京:中国林业出版社, 2004.
[2]吴春笃,王倩,储金宇.脉冲磁场处理含菌废水的实验研究［J］.水资源保护,2007,23(1):7779.
[3]杨巧绒,马海乐.强脉冲磁场的杀菌效果及对食品品质的影响［J］.微波学报,2004,20(3):8285.
[4]刘国民,贾建治.电磁场对微生物影响的研究［J］.曲阜师范大学学报,2002,28(2):8788.
[5]Guy J, Hallman Q, Howard Zhang. Inhibition of fruit fly (Diptera: Tephritidea) development by pulsed electric field［J］. Florida Entomologist, 1997,80(2): 239248.
[6]姜武峰,付文忠.应用KW种子仓储杀虫活化处理机防治储粮害虫生产试验报告［J］.粮食储藏,2003,32(1):1618.
[7]Cain C A. A theoretical basis for microwave and R F field effects on excitable cellular membrance［J］.IEEE Trans Microwave Theory Tech,1980,(MTT-28):142147.
[8]张小云.磁场对细胞生长分裂的影响及其机制的探讨［J］.中国科学:B辑,1989(2):164170.
[9]张小云.磁场的细胞效应研究［J］.基础医学与临床,1994,14(5):1519.
[10]陈文团,陈小帆.辐照处理对木质包装材料中松墨天牛幼虫发育的影响［J］.核农学报,2006,20(6):531534.
[11]张心团,赵和平.墨天牛生物学特性的研究进展［J］.安徽农业大学学报,2004,31(2):156157.
[12]武汉大学.分析化学实验［M］.北京:高等教育出版社,1985.
[13]程新胜,魏重生.不同虫态的烟草甲对磷化氢的敏感性及其电导率的测定［J］.中国烟草科学,2004(1):4648.
[14]索风梅,林长春.松墨天牛纤维素酶的研究［J］.林业科学研究,2004,17(5):583589.
[15]吴明,丁中文.纤维素酶抑制剂对松墨天牛消化生理的影响［J］.浙江林业科技,2003,23(1):14.
[16]郑保有，鲍立友，郑毓.热处理对松褐天牛生命力的影响［J］.浙江林业科技，2001，21（1）：4041.
[17]邹绮.植物生理学实验指导［M］.北京:中国农业出版社,1995.
基金
收稿日期：2008-04-16修回日期：2008-09-10 基金项目：国家自然科学基金资助项目（30671687）作者简介：宁少华（1983—），硕士生。*梁军(通讯作者)，研究员，研究方向为有害生物生态控制。Email:liangjun@forestry.ac.cn 引文格式：宁少华,张星耀,屈智魏.脉冲磁场对松褐天牛幼虫存活和发育的影响［J］.南京林业大学学报：自然科学版，2009,33(1):1519.
PDF(684692 KB)
