长木蜂蜂粮粗提液抑菌活性的研究

doi:10.3969/j.jssn.1000-2006.2009.02.005

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2009, Vol. 33 ›› Issue (02): 17-.doi: 10.3969/j.jssn.1000-2006.2009.02.005

长木蜂蜂粮粗提液抑菌活性的研究

贺春玲1，嵇保中1*，刘曙雯2

1.南京林业大学森林资源与环境学院，江苏南京210037；2.南京中山陵园管理局，江苏南京210014

出版日期:2009-04-18 发布日期:2009-04-18
基金资助:
收稿日期：2008-09-02修回日期：2008-12-01基金项目：南京林业大学“十五”人才工程项目(PD2005-01)作者简介：贺春玲(1974—)，博士生。*嵇保中(通讯作者)，教授，研究方向为森林昆虫学。Email: jbz9885@njfu.com.cn引文格式：贺春玲,嵇保中,刘曙雯. 长木蜂蜂粮粗提液抑菌活性的研究[J]. 南京林业大学学报:自然科学版,2009,33(2):17-21.

Study on the antibacterial activity of the raw extraction from bee bread of Xylocopa tranquebarorum

HE Chunling1, JI Baozhong1*, LIU Shuwen2

1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.Management Office of Sun Yatsen’s Mausoleum, Nanjing 210014, China

Online:2009-04-18 Published:2009-04-18

摘要/Abstract

摘要： 采用不同溶剂提取长木蜂蜂粮粗提液，并用杯碟法测定所得粗提液的抑菌活性。结果表明：不同的浸提方法提取粗提液得率有明显差别，沸水提取的得率最高，为80.14%；乙醚提取得率最低，为22.96%。不同溶剂的粗提液抑菌活性以70%(质量分数)乙醇粗提液的抑菌活性最强，水提液的次之，沸水提取液的最低。70%乙醇粗提液对细菌的抑菌活性明显大于对真菌的抑菌活性，对不同指示菌的抑菌活性有显著差异，表现为抑菌活性随粗提液浓度梯度增加而增强。粗提液对各种细菌的抑菌活性从高到低依次为：枯草杆菌、鳗弧菌、金黄色葡萄球菌、大肠杆菌、耶尔森菌。其中，对枯草芽孢杆菌最大抑菌圈直径为(23.61±0.89)mm。

Abstract: Crude extracts of bee bread from Xylocopa tranquebarorum were obtained using different solutions. The antibacterial activities of these extracts were investigated by using oxford plate. The results showed that there were significant differences of recovery ratio among different extraction methods. The extraction with boiling water showed the highest recovery ratio of 80.14%, while the extraction with aether showed the lowest recovery ratio of 22.96%. There were also differences of antibacterial activity among different crude extraction. Extract by 70% ethanol extracts showed the highest antibacterial activity, followed by distilled water extracts, the boiling water extracts showed the lowest antibacterial activity. The antibacterial activity of ethanol extracts on bacteria was significantly greater than that on fungi. Also, the antibacterial activity on different bacteria was significantly different. The antibacterial activity on deferent bacterium was as follow: Bacillus subtilis, Vibrio anguillarum, Staphylococcus aureus, Escherichi acoli, Yersiniaen terocolitica. The maximal inhibition diameter to Bacillus subtilis was (23.61±0.89) mm.

中图分类号:

S896.9

贺春玲,嵇保中,刘曙雯. 长木蜂蜂粮粗提液抑菌活性的研究[J]. 南京林业大学学报（自然科学版）, 2009, 33(02): 17-.

HE Chunling1, JI Baozhong1*, LIU Shuwen2. Study on the antibacterial activity of the raw extraction from bee bread of Xylocopa tranquebarorum[J].Journal of Nanjing Forestry University (Natural Science Edition), 2009, 33(02): 17-.DOI: 10.3969/j.jssn.1000-2006.2009.02.005.

参考文献

[1]吴燕如. 中国经济昆虫志. 膜翅目,蜜蜂总科:第九册[M]. 北京:科学出版社,1965.
[2]嵇保中,刘曙雯,徐丽娜,等. 蜂粮研究进展[J]. 中国农学通报,2006,22(8):165-169.
[3]苏松坤. 蜂粮的酿制机理和营养价值的研究[D]. 杭州:浙江大学,2000.
[4]Nagai T, Nagashima T, Myoda T, et al. Preparation and functional properties of extracts from bee bread[J]. Nahrung, 2004, 48(3): 226-229.
[5]Nagai T, Nagashima T, Suzuki N, et al. Antioxidant activity and angiotensin I-converting enzyme inhibition by enzymatic hydrolysates form bee bread[J]. Z Naturforsch, 2005, 60(1-2): 133-138.
[6]Baltrusaityte V, Venskutonis P R, Ceksteryte V. Radical scavenging activity of different floral origin honey and beebread phenolic extracts[J]. Food Chemistry, 2007, 101(2): 502-514.
[7]袁耀东. 蜂粮的成分和作用[J]. 中国养蜂,1991(6):36-37.
[8]张永华. 漫谈花粉粒花粉团花粉脾和蜂粮脾[J]. 中国养蜂,2000,51(5):12.
[9]Egorova A I. Preservative microflora in stored pollen[J]. Veterinariya, 1971(8): 40-41.
[10]Gilliam M , Prest D B, Lorenz B J. Microbiology of pollen and bee bread: taxonomy and enzymology of molds[J]. Apidologie, 1989, 20: 53-68.
[11]苏松坤,陈盛禄,余旭平. 蜂粮酿制过程中细菌的变化规律[J]. 浙江大学学报:农业与生命科学版,2002,28(5):575-577.
[12]苏松坤,陈盛禄. 茶蜂花粉及其蜂粮的营养成分研究[J]. 上海交通大学学报:农业科学版,2002,20(2):95-99.
[13]Fujiwara S, Imai J , Fujiwara M , et al. A potent antibacterial protein in royal jelly: Purification and determination of the primary structure of royalisin[J]. Journal of Biological Chemistry, 1990, 265(19): 11333-11337.
[14]Gillian M. Microbiology of pollen and bee bread: the genus bacillus[J]. Apidologic, 1979, 10(3): 269-275.
[15]吴春如. 中国动物志. 昆虫纲:第二十卷[M]. 北京:科学出版社,2000.
[16]罗禄怡,罗次毕,谢继石,等. 红三叶草种籽场长木蜂的人工训育及利用研究[J]. 草业科学,1992,9(6):5-8.
[17]周德庆. 微生物学实验手册[M]. 上海:上海科学技术出版社,1986.
[18]胡春水. 食疗保健话蜂粮[J]. 中国食品,2000,13:15.
[19]苏松坤,陈盛禄,林雪珍,等. 茶蜂花粉蜂粮中花粉形态和营养成分的比较[J]. 中国养蜂,2000,51(6):3-6.
[20]郭芳彬. 蜂胶的抗菌作用[J]. 蜜蜂杂志,2004(3):10-12.
[21]申慧亭,靳月琴. 蜂胶抗菌作用的实验室研究[J]. 长治医学院学报,2005,19(4):253-254.

长木蜂蜂粮粗提液抑菌活性的研究

Study on the antibacterial activity of the raw extraction from bee bread of Xylocopa tranquebarorum

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价

作者

读者

审稿