采用海藻酸钙凝胶包埋固定小球藻，对人工污水进行静态模拟净化试验，研究了不同pH条件下铜对固定化小球藻去除污水中氨氮和硝酸氮能力的影响。结果表明：有铜存在时第5天，pH为4、5、6、7、8时固定化小球藻的NH+3N的去除率相对无铜条件分别下降了4.0%、0.6%、5.9%、6.0%和4.4%，悬浮态小球藻的NH+3N的去除率分别下降了10.9%、22.4%、15.5%、14.5%和20.2%；pH为6、7、8时固定化小球藻的NO-3N的去除率相对无铜条件分别下降了4.1%、2.8%和4.2%，但pH为4、5时固定化小球藻的NO-3N的去除率却上升了0.8%和02%，在pH为4、5、6、7、8时悬浮态小球藻的NO-3N的去除率相对无铜条件则分别下降了4.4%、92%、5.3%、10.6%和10.2%。总体而言，铜抑制了小球藻的除氮能力，尤其对悬浮态小球藻的除氮能力产生了较大的抑制性，而固定化处理在一定程度上减弱了铜对小球藻除氮能力的影响。
Abstract
The static simulation test for synthetic wastewater purification was carried out by using Chlorella pyrenoidosa entrapped in calcium alginate, and influence of copper at different pH on the nitrogenremoval efficiencies of immobilized Chlorella pyrenoidosa for ammonia and nitric acidcontaining wastewater were also investigated. Test results showed that comparing without copper in wastewater, when copper was added，the NH+3N removal of immobilized Chlorella pyrenoidosa in 5 days at pH=4, 5, 6, 7, 8 was reduced 4.0%, 0.6%, 5.9%, 6.0% and 4.4% respectively, the NH+3N removal of suspended Chlorella pyrenoidosa was reduced 10.9%, 22.4%, 15.5%, 14.5%, 20.2% respectively; The NO-3N removal of immobilized Chlorella pyrenoidosa at pH=6, 7, 8 was reduced 4.1%, 2.8% and 4.2% respectively, but the NO-3N removal at pH=4, 5 was increased 0.8%, 0.2%, the NO-3N removal of suspended Chlorella pyrenoidosa was reduced 4.4%, 9.2%, 5.3%, 10.6%, 10.2% respectively. So copper has inhibited the nitrite uptake efficiency of Chlorella pyrenoidosa in free cells than the immobilized ones, and the treatment of immobilization to algae smoothed the disruption of copper.
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基金
收稿日期：2008-11-10修回日期：2009-03-31基金项目：国家“十一五”科技支撑计划(2006BAD03A19)；江苏省林业生态工程重点实验室开放研究课题资助项目；南京林业大学大学生科技创新基金资助项目作者简介：李川(1972—)，讲师，博士生，研究方向为环境生态。Email: lichuan_2001@hotmail.com。引文格式：李川,薛建辉,苏莹莹,等. 不同pH条件下铜对固定化小球藻除氮效果的影响[J]. 南京林业大学学报:自然科学版,2009,33(4):105-108.
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