荧光假单胞菌Pseudomonas fluorescens GcM51A菌株的胞外木素过氧化物酶的部分纯化和鉴定

孔令营; 郭道森; 赵博光; 李荣贵

doi:10.3969/j.jssn.1000-2006.2010.02.036

孔令营，郭道森，赵博光，李荣贵

南京林业大学学报（自然科学版） ›› 2010, Vol. 34 ›› Issue (02) : 151-152.

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南京林业大学学报（自然科学版） ›› 2010, Vol. 34 ›› Issue (02) : 151-152. DOI: 10.3969/j.jssn.1000-2006.2010.02.036

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荧光假单胞菌Pseudomonas fluorescens GcM51A菌株的胞外木素过氧化物酶的部分纯化和鉴定

孔令营¹，郭道森¹，赵博光²，李荣贵¹

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Partial purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM51A

KONG Lingying¹, GUO Daosen¹, ZHAO Boguang², LI Ronggui¹

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摘要

荧光假单胞菌GcM51A是松材线虫携带的一种致病菌。在GcM51A的培养液中发现有木质素过氧化物酶的活性，同时显示很强的毒性。通过对其胞外木质素过氧化物酶的酶学性质的初步研究发现：其最适温度为35 ℃，最适pH为4.0。在15~35 ℃、pH为6.0~9.0范围内，酶活保持相对稳定。NH2OH·HCl和KCN对酶活的抑制作用分别达到88 %和57 %，而NaN3对酶活的抑制作用只有15 %左右。Ca2+、Mn2+ 和Fe3+对其活性有增强作用，但是Hg2+ 和 Zn2+却有中等的抑制作用。KCN 和NH2OH·HCl抑制其酶活性分别达57 %、88 %，而NaN3的抑制率仅为15 %。吸收光谱显示该酶在406 nm处有一个Soret峰，加入1 mmol/L Na2S2O4后，吸光度出现了明显的下降，但Soret峰没有显著移动。

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孔令营，郭道森，赵博光，李荣贵. 荧光假单胞菌Pseudomonas fluorescens GcM51A菌株的胞外木素过氧化物酶的部分纯化和鉴定[J]. 南京林业大学学报（自然科学版）. 2010, 34(02): 151-152 https://doi.org/10.3969/j.jssn.1000-2006.2010.02.036

KONG Lingying, GUO Daosen, ZHAO Boguang, LI Ronggui. Partial purification and characterization of extracellular lignin peroxidase from Pseudomonas fluorescens GcM51A[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2010, 34(02): 151-152 https://doi.org/10.3969/j.jssn.1000-2006.2010.02.036

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