Abstract: The regression mathematical model was established on the basis of transmittancy of clarified ginkgo juice and optimum enzymolysis condition were determined according to the result of variance analysis to the experimentation. The results showed that the influence order of four factors on transmittancy of ginkgo juice from high to low was: neutral proteinase, enzymolysis temperature, maltogenic amylase, αamylase. Thereinto, interactions between αamylase and enzymolysis temperature, neutral proteinase and maltogenic amylase, maltogenic amylase and enzymolysis temperature have appreciable impacts on the transmittancy of clarified ginkgo juice. The optimal technology parameters: αamylase, neutral proteinase, maltogenic amylase, enzymolysis temperature are 0.09 %, 0.113 %, 0.078 %, and 63.5 ℃, respectively. Under these conditions, the transmittancy of clarified ginkgo juice can reach 65.88 % while the verified value is 65.65 %, and this is obviously higher than that before enzyme treatment.