Abstract: To explore the impact of ectomycorrhizal fungi on photosynthesis of the host plant, chlorophyll content and chlorophyll fluorescence parameters changes in three kind of pine seedlings including Pinus massoniana, P.elliottii, and P.thunbergii, respectively treated with Boletus edulis, Boletus speciosus, Pisolithus tinctorius, Rhizopogen luteous, Laccaria aemthystea and Amanita pantherina were investigated. The results indicated that inoculating with ectomycorrhizal fungi obviousely increased chlorophyll contents, actal photochemical efficiencies of PS Ⅱ (ФPSⅡ) and electron transfer rates (ETR) in needles. In three kind of pine seedlings, chlorophyll content and actal photochemical efficiency of PS Ⅱ showed similar changes in different inoculation treatments, and they both were higher in those seedlings inoculated with B.edulis, B.speciosus, and P.tinctorius. For PS Ⅱ electron transfer rate, the highest was L.aemthystea treatment in P.massoniana and P.thunbergii seedlings, and was P.tinctorius treatment in P.elliottii seedlings. The chlorophyll content showed significant positive correlation with actal photochemical efficiencies of PS Ⅱ in three kind of pine seedlings.