南京林业大学学报(自然科学版) ›› 2011, Vol. 35 ›› Issue (01): 6-10.doi: 10.3969/j.jssn.1000-2006.2011.01.002

• 研究论文 • 上一篇    下一篇

松属GAs相关ESTSSR标记与火炬松×洪都拉斯加勒比松苗高相关性分析

栾启福,姜景民,张守攻   

  1. 1. 中国林业科学研究院亚热带林业研究所,浙江富阳311400;2. 中国林业科学研究院林业研究所,北京100091
  • 出版日期:2011-01-14 发布日期:2011-01-14
  • 基金资助:
    收稿日期:2010-05-26修回日期:2010-11-06基金项目:浙江省科技计划项目(2008C32015);国家农业科技成果转化资金项目(2009GB24320476)作者简介:栾启福(1976—),助理研究员,博士。*姜景民(通信作者),研究员。Email:Jmjiang6001@126.com。

Analysis on correlation of ESTSSR markers relating to GAs metabolism for Pinus L. with the seedling height of Pinus taeda×P. caribaea var. hondurensis

LUAN Qifu, JIANG Jingmin, ZHANG Shougong   

  1. 1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400,China;2. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091,China
  • Online:2011-01-14 Published:2011-01-14

摘要: 以火炬松×洪都拉斯加勒比松F1代群体为研究对象,从松树PGI(松类基因索引)数据中筛选出13个与赤霉素(GAs)代谢有关的序列。设计了这13条序列的ESTSSR引物对,并筛选出4对引物作为F1代检测的较好的标记。4对引物PCR分析显示在2个亲本和39个子代中共扩增出1 014个多态性位点,其中,杂种F1代扩增出的位点数中有50.19 %与父本相同,5217 %与母本相同,这表明母本(火炬松)和父本(加勒比松)杂交能够得到获得双亲遗传物质的新杂种。4个引物检测的26个等位基因位点中有6个与苗龄6个月的苗高有显著或极显著的相关性,有5个位点与苗龄9个月的苗高有显著或极显著相关性。这为早期选择提供了较好的分子标记。

Abstract: Thirteen ESTSSR molecular markers related to GAs metabolism for Pinus L. were developed from EST sequences in PGI(Pinus gene index)database. 1 014 alleles were amplified with two parents and 39 F1 progeny of Pinus taeda×P. caribaea var. hondurensis with four primerpairs, which were selected from the thirteen ESTSSRs. 5019 % of the 1 014 alleles were originated from the male parent(P. taeda) and 5217 % were shared with the female parent(P. caribaea var. hondurensis), which showed that the progeny of this hybridization inherent genetic materials from both parents. Six locus selected from all 26 locus amplified by four primer pairs were related significantly to sixmonth old seedling height of the 39 F1 hybrids, and five locus related significantly to ninemonth old seedling height. Therefore, this markers can serve as good molecular markers for early selection.

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