南京林业大学学报(自然科学版) ›› 2012, Vol. 36 ›› Issue (02): 47-51.doi: 10.3969/j.jssn.1000-2006.2012.02.010

• 研究论文 • 上一篇    下一篇

油茶基因组微卫星特征分析

史 洁,尹佟明,管宏伟, 戴晓港,陈金慧,施季森*   

  1. 南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,江苏 南京 210037
  • 出版日期:2012-04-10 发布日期:2012-04-10
  • 基金资助:
    收稿日期:2011-09-08 修回日期:2011-12-12 基金项目:国家林业局林业公益性行业科研专项项目(200904002) 第一作者:史洁,博士生。*通信作者:施季森,教授。E-mail: jshi@njfu.edu.cn。引文格式:史洁,尹佟明,管宏伟,等. 油茶基因组微卫星特征分析[J]. 南京林业大学学报:自然科学版,2012,36(2):47-51.

Characteristic analysis of microsatellites of Camellia spp.

SHI Jie, YIN Tongming, GUAN Hongwei, DAI Xiaogang, CHEN Jinhui, SHI Jisen*   

  1. Key Lab of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037,China
  • Online:2012-04-10 Published:2012-04-10

摘要: 对油茶基因组约10%覆盖度的DNA序列进行微卫星查找,共获得11 344个重复单元长度为1~6碱基的微卫星。在此基础上,通过对这些微卫星序列分析发现:在油茶基因组中长度为二核苷酸的微卫星重复单元最为丰富,占27.1%; 在单碱基重复和二碱基重复这两种类型中,最主要的优势重复单元分别是A/T以及AT/TA、AG/TC。三碱基、四碱基、五碱基重复类型中,(AAN)n、(AAAN)n和(AAAAN)n为对应的优势重复单元,这些优势重复单元中富含碱基A和T。油茶基因组中变异程度高的微卫星(长度≥20 bp)约占11.7%。分析还发现,除单核苷酸重复微卫星外,油茶基因组微卫星长度的变异速率与重复单元长度呈负相关,即油茶基因组中长度较短的微卫星变异速率较快,而较长的重复单元变异速度较慢,相对较为稳定。

Abstract: There were 11 344 microsatellites(SSRs)ranged from 1 to 6 bp detected by analyzing the DNA sequences covering about 10% of genome Camellia. Among these SSRs, dinucleotide repeats were most abundant(27.1%). For the composition of microsatellites, A/T repeats were the most frequent motifs in mononucleotide repeats, and AT/TA, AG/TC repeats were the most in dinucleotide repeats, while(AAN)n,(AAAN)n and(AAAAN)n repeats were dominant in tri-, tetra- and pentanucleotide repeats, respectively. All the dominant repeat motifs for different type of SSRs were abundant in nucleotides A and T. In Camellia genome, microsatellites longer than 20bp accounted for about 11.7% of the detected SSRs. Repeats, repeat motif lengths were found negatively correlated with the variation rate except mononucleotide repeats, thus, the shorter repeats would have faster substitution rate.

中图分类号: