对不同含水率的金钱松种子在贮藏期间的发芽率、生活力以及各项生理生化指标的变化进行分析,结果表明:(1)金钱松种子临界含水率在3%～4%。含水率与发芽率、生活力以及POD活性之间存在显著负相关性,相关性大小排序为生活力、POD活性、发芽率。(2)金钱松种子可溶性蛋白质含量、粗脂肪含量、MDA含量、游离脯氨酸含量、SOD和POD活性与发芽率、生活力之间存在显著负相关性; 各项指标与发芽率的相关性大小排序为SOD活性、MDA含量、可溶性蛋白质含量、游离脯氨酸含量、POD活性、粗脂肪含量,与种子生活力的相关性大小排序为SOD活性、POD活性、游离脯氨酸含量、粗脂肪含量、蛋白质含量、MDA含量。(3)O</sup><sup>-· 2含量虽然与金钱松种子发芽率、种子生活力之间的相关性不显著,但是与种子的可溶性糖和淀粉存在显著负相关性、与MDA含量之间存在显著正相关性。(4)金钱松种子可溶性糖和淀粉的含量与其他生理生化指标之间的相关性不显著。
Abstract
A study was conducted on the germination, seed viability and some physiological and biochemical indices of Pseudolarix kaempferi seeds collected from Arboretum of Nanjing Forestry University on October 24, 2009 under the condition of seed preservation. The results were described as follows:(1)Critical humidity was 3%-4%. Germination rate, seed viability and POD activity these 3 indices each exists a significant negative correlation with moisture content. The relativity were ordered in a descending row as seed viability, POD activity and germination rate.(2)Soluble protein, crude fat, MDA content, free proline content, SOD activity and POD activity these 6 indices each exists a significant negative correlation with germination rate and seed viability. Relativity of correlation between germination rate and these 6 indices were ordered in a descending row as SOD activity, MDA content, soluble protein, free proline content, POD activity and crude, and the relativity of correlation between seed viability and these 6 indices were ordered in a descending row as SOD activity, POD activity, free proline content. crude fat, soluble protein and MDA content.(3)Negative correlation were found between O</sup><sup>-· 2 content and soluble sugar and also starch content, while positive correlation between O</sup><sup>-· 2 content and MDA content were significant.(4)No significant correlation was found between soluble sugar and starch content these 2 indices and other physiological and biochemical indices.
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基金
收稿日期:2011-01-15 修回日期:2011-05-08 基金项目:教育部高等学校博士学科点专项科研基金项目(20113204110010) 第一作者:廖文燕,博士生。*通信作者:高捍东,教授。E-mail:gaohd@njfu.edu.cn。引文格式:廖文燕,高捍东. 金钱松种子贮藏过程中的生理生化特征研究[J]. 南京林业大学学报:自然科学版,2012,36(2):52-58.
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