以蛹蜂比为1:100和柞蚕(Antheraea pernyi Guerin-Meneville)蛹为替代寄主繁殖白蛾黑基啮小蜂(Tetrastichus nigricoxae Yang),测定室内人工繁蜂的最适交配期和接蜂暴露期。结果表明:当小蜂羽化出蜂后交配48~72 h时,寄生率(93.3%~94.2%)、羽化率(87.5%~90.8%)和平均子代出蜂量(1 010.4~1 213.3头)等寄生指标,均高于或显著高于24 h时的值(p<0.05),极显著高于0 h和96~144 h时(p<0.01)的值; 当寄主蛹暴露给寄生蜂2~3 d时,寄生率(87.5%~95.8%)、羽化率(86.7%~95.8%)和平均子代出蜂量(1 274.5~1 365.6头)等繁蜂指标也高于或显著高于其他暴露期处理(p<0.05)。综合考虑蜂龄对寄生效果的影响,选择白蛾黑基啮小蜂人工繁殖的最适交配期和暴露期分别为羽化出蜂后2 d和2~3 d。
Abstract
Artificial production experiments were made to determine the optimum period of mating and exposure for the laboratory production of Tetrastichus nigricoxae with Antheraea pernyi pupae as hosts(host pupa: parasitoid ratio=1:100). The results showed that when the mating time of wasp 48-72 h after exiting the host pupae, parasitism capability such as parasitism rate(93.3%-94.2%), eclosion rate(87.5%-90.8%)and average number of offspring per host(1 010.4-1 213.3)were significantly higher than that of wasp which mating time were 24 h(p<0.05), 0 h and 96-144 h(p<0.01). The parasitism(87.5%-95.8%), eclosion(86.7%-95.8%)and average number of offspring(1 274.5-1 365.6)were significantly higher than other exposure time(p<0.05). When pupae were exposure for 2-3 d. Considering the effect of wasp age to parasitism, the optimum period of mating and exposure for the laboratory production were 2 d after exiting the host pupae and 2-3 d respectively.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 颜学武,郭同斌,蒋继宏,等.白蛾黑基啮小蜂的生物学特性[J].南京林业大学学报:自然科学版,2008,32(6):29-33.
[2] Wu Z X, Nordlund D A. Superparasitism of Lygus hesperus Knight eggs by Anaphes iole Girault in the laboratory [J]. Biological Control, 2002, 23(2): 121-126.
[3] 颜学武,郭同斌,蒋继宏,等.白蛾黑基啮小蜂寄主接受行为的研究[J].南京林业大学学报:自然科学版,2009,33(5):121-125.
[4] 郭同斌,王虎诚,徐克勤,等.白蛾黑基啮小蜂的人工繁殖及其对杨小舟蛾的防治效果[J].南京林业大学学报:自然科学版,2010,33(5):81-86.
[5] 黄山春,覃伟权,周焕起,等.椰心叶甲啮小蜂的繁殖生物学研究[J].华东昆虫学报,2007,16(3):168-171.
[6] 高惠璇,李东风,耿直,等.SAS系统与基础统计分析[M].北京:北京大学出版社,1995.
[7] Arun-Baitha, Jalali S K, Rabindra R J, et al. Parasitising efficiency of the pupal parasitoid, Tetrastichus howardi(Olliff)(Hymenoptera: Eulophidae)on Chiol partellus(Swinhoe)at different exposure periods[J]. Journal of Biological Control, 2004, 18(1):65-67.
基金
收稿日期:2010-11-11 修回日期:2011-04-08 基金项目:江苏省“333工程”科研项目(BRA2010027); 江苏省林业三项工程项目(lysx(2009)08); 徐州市科技项目(XF10C001) 第一作者:郭同斌,研究员级高级工程师,博士。E-mail:gtb1101@163.com。引文格式:郭同斌,梁波,杨中林,等. 人工繁殖白蛾黑基啮小蜂最适交配期和暴露期的选择[J]. 南京林业大学学报:自然科学版,2012,36(2):73-76.