采用四唑染色法、靛蓝染色法以及碘化钾反应法(I-KI)对檀香(Santalum album)种子生活力进行测定,分析了四 唑的质量分数、染色温度以及染色时间对檀香种子生活力测定的影响,并将各种不同生活力测定方法的测定结果与发芽试验结 果进行了比较分析。结果表明,四唑法测定檀香种子生活力的最佳测定条件为使用质量分数为0.1%的四唑,在40 ℃染色3 h。 四唑的质量分数(p<0.001)、染色温度(p=0.006)以及质量分数和染色温度之间的交互作用 (p=0.001)对檀香种子生活力的测定结果均有显著影响。不同的生活力测定方法之间差异显著(p<0.001), 其中以四唑法测定结果与发芽率接近,是檀香种子生活力快速测定的理想方法。靛蓝染色法和碘化钾反应法均不适合用作檀香 种子生活力的快速测定。
Abstract
In order to find the most suitable method for rapid determination of seed viability of sandal (Santalum album), TTC method, indigo dying method as well as I-KI reaction method were studied by comparing with the germination test. The results showed that the best testing condition for sandal seed viability determination was staining 3 h with 0.1% TTC at 40 ℃ under darkness. Significant differences were found among TTC concentration(p<0.001), staining temperature(p=0.006)and the interaction between TTC concentration and staining temperature(p=0.001), great differences also existed among different testing methods(p<0.001). TTC method got the most similar results with the germination rate, and would be the most suitable method for rapid determination of seed viability of sandal, while indigo dying method and I-KI reaction method were not suitable.
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基金
收稿日期:2011-09-19 修回日期:2012-03-28 基金项目:国家林业局“948”项目(2008-04-02); 广东省林业局林业科技创新与示范专项项目(2010KJCX002-01); 科技部农 业科技成果转化资金项目(2011GB24320004); 国家林业公益性行业科研专项项目(201204301) 第一作者:刘小金,博士。*通信作者:徐大平,研究员。E-mail: gzfsrd@pub.guangzhou.gd.cn。引文格式:刘小金,徐大平,杨曾奖,等. 檀香种子生活力快速测定法[J]. 南京林业大学学报:自然科学版,2012,36(4):67-70.
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