采用流式细胞术研究不同引发处理下马尾松种子胚细胞DNA含量的差异，测定不同引发处理下马尾松种子的发芽率。结果表明：（1）PEG 8000溶液引发处理能促进胚细胞DNA的复制，4倍体细胞的比例增加，其中以20 %PEG 8000溶液引发3～4 d的处理对种子胚细胞DNA合成的促进作用最明显。（2）胚细胞增殖指数最高的引发处理是20 %PEG 8000溶液在15 ℃时引发3～4 d。（3）较高浓度的PEG 8000溶液和较长的引发时间有利于提高马尾松种子发芽率。（4）在一定范围内，胚细胞增殖指数与种子发芽率呈显著正相关。
Abstract
In order to reveal the cytological mechanism of promoting seed germination through seed priming, the differences of DNA content in embryonic cell of Pinus massoniana seeds after different priming treatments were studied by using flow cytometry, and seed germination percentage was also determined. The results showed that: (1) DNA replication in embryonic cell could be enhanced by PEG 8000 solution pretreatment, the ratio of tetraploid cells increased, the best treatment was a regime treated for 3-4 days using 20 % PEG 8000 solution. (2) The best priming treatment for increasing cell proliferation index was a regime treated in 20 % PEG 8000 solution for 3-4 days at 15 ℃. (3) Higher PEG 8000 concentration and longer pretreatment time were benefit for increasing germination percentage. (4) In a certain extent, there was a significant positive correlation between the cell proliferation index and seed germination percentage.
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参考文献
[1]郑光华. 种子生理研究[M]. 北京: 科学出版社，2004.
[2]焦旭雯, 赵树进. 流式细胞术在高等植物研究中的应用[J]. 热带亚热带植物学报, 2006，14(4): 354-358.
[3]Sliwinska E. Nuclear DNA replication and seed quality [J]. Seed Science Research, 2009，19（1）: 15-25.
[4]国家质量技术监督局. GB 2772—1999 林木种子检验规程[S]. 北京：中国标准出版社，2000.
[5]吴后男.流式细胞术原理与应用教程[M].北京：北京大学医学出版社，2008.
[6]翟中和，王喜忠，丁明孝.细胞生物学[M]. 3版.北京：高等教育出版社，2007.
[7]Bino R J, de Vries J N, Kraak H L, et al. Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination [J]. Annals of Botany，1992，69(3): 231-236.
[8]Lanteri S, Saracco F, Kraak H L, et al. The effects of priming on nuclear replication activity and germination of pepper (Capsicum annuum) and tomato (Lycopersicon esculentum) seeds[J]. Seed Science Research，1994，4(2):81-87.
[9]Saracco F, Bino R J, Bergervoe J H V, et al. Influence of priminginduced nuclear replication activity on storability of pepper (Capsicum annuum L.) seeds [J]. Seed Science Research，1995，5(1): 25-29.
[10]Van Pijlen J G, Groot S P C, Kraak H L,et al. Effects of prestorage hydration treatments on germination performance, moisture content, DNA synthesis and controlled deterioration tolerance of tomato (Lycopersicon esculentum Mill.) seeds [J]. Seed Science Research，1996，6(2): 57-63.
[11]Gurusinghe S H, Cheng Z, Bradford K J. Cell cycle activity during seed priming is not essential for germination advancement in tomato [J]. Journal of Experimental Botany，1999，50(330): 101-106.
[12]De Castro R D, van Lammeren A A M, Groot S P C, et al. Cell division and subsequent radicle protrusion in tomato seeds are inhibited by osmotic stress but DNA synthesis and formation of microtubular cytoskeleton are not [J]. Plant Physiology，2000，122(2): 327-335.
[13]Sliwinska E, Jendrzejczak E. Sugarbeet seed quality and DNA synthesis in the embryo in relation to hydrationdehydration cycles [J]. Seed Science and Technology，2002，30(3): 597-608.
[14]Sliwinska E, Sadowski H. Comparison of the effects of bioactive compounds and hydration treatments on germination and cell cycle activity of sugarbeet seeds [J]. Seed Science and Technology，2004，32(3):847-850.
[15]Kozeko L E, Troyan V M. The relationship between the mitotic activity and moisture content of recalcitrant seeds of Acer saccharinum(L.) during maturation, postmaturation drying and germination[J]. Seed Science Research，2000，10(3): 225-232.
[16]Dahal P, Bradford K J, Jones R A. Effect of priming and endosperm integrity on seed germination rates of tomato genotypes. I: Germination at suboptimal temperature [J]. Journal of Experimental Botany, 1990, 41(11):1441-1453.
[17]Taylor A G, Motes J E, Kirkham M B. Germination and seedling growth characteristic of three tomato species affected by water deficits[J]. Journal of the America Society for Horticultural Science, 1982, 107(1):282-285.
[18]De R, Kar R K. Seed germination and seedling growth of mungbean (Vigna radiata) under water stress induced by PEG6000 [J]. Seed Science & Technology, 1995, 23(2):301-308.
[19]Chamberlin M A, Horner H T, Palmer R G. Nuclear size and DNA content of the embryo and endosperm during their initial stages of development in Glycine max (Fabaceae)[J]. American Journal of Botany, 1993, 80(10): 1209-1215.
[20]Wyman J, Tremblay M F, Laliberte S. Cell cycle activation during imbibition and visible germination in embryos and megagametophytes of Jack pine (Pinus banksiana Lamb.)[J]. Annals of Botany, 1996, 78(2): 245-253.
基金
收稿日期：2011-12-04修回日期：2012-05-25
基金项目：江苏省普通高校研究生科研创新计划资助项目（苏教研〔2009〕8号）
第一作者：任淑娟，硕士生。*通信作者：喻方圆，教授。E-mail: fyyu@njfu.edu.cn。
引文格式：任淑娟，喻方圆，徐丽萍. 引发对马尾松种子细胞周期和发芽率的影响[J]. 南京林业大学学报:自然科学版,2012,36(5):51-55.
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