南京林业大学学报(自然科学版) ›› 2012, Vol. 36 ›› Issue (06): 135-139.doi: 10.3969/j.jssn.1000-2006.2012.06.027

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环介导等温基因扩增技术在林木病害检测中的应用展望

邱秀文,叶建仁*,张林平   

  1. 南京林业大学森林资源与环境学院,江苏省有害生物入侵预防与控制重点实验室,江苏南京210037
  • 出版日期:2012-11-30 发布日期:2012-11-30
  • 基金资助:
    收稿日期:2012-03-20修回日期:2012-08-28
    基金项目:国家林业局推广项目(2011-51)
    第一作者:邱秀文,博士生。 *通信作者:叶建仁,教授。 Email: jrye@njfu.com.cn。
    引文格式:邱秀文,叶建仁,张林平. 环介导等温基因扩增技术在林木病害检测中的应用展望[J]. 南京林业大学学报:自然科学版,2012,36(6):135-139.

The application prospect of loopmediated isothermal amplification method in forest disease detection

QIU Xiuwen, YE Jianren*, ZHANG Linping   

  1. Jiangsu Key Laboratory for Prevention and Management of Invasive Specie, College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
  • Online:2012-11-30 Published:2012-11-30

摘要: 森林病害主要是由病毒、细菌、真菌和寄生性生物引起的,其中真菌所致病害种类最多,约占森林病害的80 %以上。环介导等温基因扩增(LAMP)技术是一种快速高效的DNA扩增方法,具有简单、快捷、特异性强、准确性高等特点,笔者论述了LAMP技术的原理及其在病毒、细菌、真菌和寄生性生物等方面的应用及研究。该技术广泛应用于食品、临床、环境、农业、工业和畜牧业等领域。环介导等温扩增技术为快速分子检测提供了一条新的途径,在林木病害检测领域将会有广阔的应用前景。

Abstract: Forest diseases are mainly caused by virus, bacteria, fungi and parasitic organisms, and more than 80 % ofthem are caused by fungi. Loopmediated isothermal amplification (LAMP) is a fast and efficient method with the characteristics of simplicity, facility, and accuracy for DNA amplification. This article was focused on the principles and application of the LAMP technology. It had been widely used in the field of food, clinic, environment, agriculture, industry and animal husbandry. It will have a broad prospect in forestry molecular detection.

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