Abstract: Sciences, culture, economics, politics, and daily life was widely effected by the research progresses of life science in the 20th century. Especially in the later of 80’s in the 20th century, with the remarkable initiation and bringing into effect of Human Genome Plan and Other species genome plan, the nature science and society science was going to have more and more common joints with more new high sciences and technologies was born. Bioinformatics was one of them, by crossing and intergrating with molecular biology, computing biology, computer science and information science. The molecular data of organisms have been producing and increasing exponentially. Molecular biologists need the modern computer and network technology to organize and use those data. Bioinformatics will be playing an important role and take important effect on each species genomics, post-genomics and new drug development. Bioinformatics would be the foundation stone of life science in the 21st century. However, Bioinformatics was immature and it might be totally understood through the genetic information flow, and its storage and copying, expressing, recombination. The releted basic concept, research contents, aim and devolopment of this new subject were summarized in this paper.