南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (04): 115-120.doi: 10.3969/j.jssn.1000-2006.2007.04.026

• 研究论文 • 上一篇    下一篇

不同致病力线虫接种黑松后寄主体内组织病理学变化

金钢,叶建仁*   

  1. 南京林业大学森林资源与环境学院, 江苏 南京 210037
  • 出版日期:2007-08-18 发布日期:2007-08-18

Histopathological Study on the Nematode in Seedling of Pinus thunbergii

JIN Gang, YE Jian-ren*   

  1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China
  • Online:2007-08-18 Published:2007-08-18

摘要: <正>从200个随机引物中筛选出16个多态性稳定的引物,对38个蜡梅品种进行遗传多样性和亲缘关系RAPD分析。共扩增出154条DNA片段,其中,多态性DNA带98条,占总扩增片段的63.6%。应用Popgene32软件进行Nei相似性系数和遗传距离计算,并利用UPGMA法构建聚类树状图,把供试的38个蜡梅品种分为7个大类,聚类结果与形态分类结果基本一致。结果表明,RAPD标记可用于蜡梅品种鉴定和亲缘关系探讨。

Abstract: Two Bursaphelenchus xylophilus isolates (virulent and avirulent) and one B, mucronatus isolate were inoculated separately on 2-year-old seedlings of Pinus thunbergii. The results showed that behavior of nematode dispersal in seedlings caused by different isolates varied considerably, which caused in the development of histopathology within the host to be significantly different. The virulent isolate of B. xylophilus dispersed throughout the host via axial and radial resin duct. Nematodes had a great dispersal ability as the resin duct was a main lacunar system of pine. Population of arivulent isolate of B. xylophilus mainly dispersed into the cortical layer which led to a lower dispersal rate and killing power. Population of B. mucronatus was mainly distributed in the cortical layer and only on few occasions was detected in resin duct. They were nonpathogenicity to pine clue to the low reproduction rate and no virulence to the cell lineages of phloem, vascular cambium, ray in xylem and parenchyma in resin passage. Results also indicated that no nematode dispersal was detected in cambium of pine regardless of the inoculated isolate which was virulent or avirulent. It suggested the cell death of cambium was a bionecrosis and the phenomenon was similar to that of parenchyma cells such as epithelial cell in resin passage, resulted indirectly from nematode dispersal.

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