南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (04): 128-132.doi: 10.3969/j.jssn.1000-2006.2007.04.029

• 研究论文 • 上一篇    下一篇

3种松材线虫分子检测技术的比较分析

王焱1,3,季镭2,余本渊2,林茂松2,叶建仁1*   

  1. 1. 南京林业大学森林资源与环境学院, 江苏 南京 210037; 2. 南京农业大学植物保护学院, 江苏 南京 210095; 3. 上海市林业病虫防治检疫站, 上海 200072
  • 出版日期:2007-08-18 发布日期:2007-08-18

Comparative Analyses on Three Kinds of Molecular Detection Technique of Bursaphelenchus xylophilus

WANG Yan1,3, JI Lei2, YU Ben-yuan2, LIN Mao-song2, YE Jian-ren1*   

  1. 1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. College of Plant Protection Nanjing Agricultural University, Nanjing 210095, China; 3. Shanghai Station of Forest Pest Quarantine and Control, Shanghai 200072, China
  • Online:2007-08-18 Published:2007-08-18

摘要: <正>林分可视化系统Stand Visualization System,SVS是森林调查样地或小班水平可视化的理想工具软件,它生成的直观、立体图像,为林分生长和林业可视化的研究提供了新方法。笔者利用湿地松人工林林分调查数据,采用林分可视化系统展示了湿地松人工林林分可视化流程,详细介绍了该系统的使用方法和注意事项,并对SVS林分模拟结果进行了分析和讨论。

Abstract: A comparative study on molecular detection of Bursaphelenchus xylophilus was conducted in this paper. Specific amplification PCR, ITS-PCR-RFLP and real time PCR were used in molecular detection of B. xylophilus. According to detection time, detection accuracy and operation skills, this paper summarized following conclusions: (1) Specific amplification PCR is used widely up to now because of its simple operation and low costs; (2) ITS-PCR-RFLP can be used to identify physiological race, being more accurate than Specific amplification PCR, however it takes long time to operate, it can be used for differentiation of population of B. xylophilus and species identification; (3) Real-time PCR takes short time to operate, has a high accurate results, and it’s easy to operate. However it needs specific instruments which are very expensive. It should be better used in quarantine of B. xylophilus.

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