南京林业大学学报(自然科学版) ›› 2007, Vol. 31 ›› Issue (04): 133-135.doi: 10.3969/j.jssn.1000-2006.2007.04.030

• 研究论文 • 上一篇    下一篇

松材线虫快速检测试剂盒研制

陈凤毛1,2,叶建仁1*,吴小芹1,汤坚2   

  1. 1. 南京林业大学森林资源与环境学院, 江苏 南京 210037; 2. 安徽省林业厅, 安徽 合肥 230031
  • 出版日期:2007-08-18 发布日期:2007-08-18

Development of Quick Detection Kit for Bursaphelenchus xylophilus

CHEN Feng-mao1,2, YE Jian-ren1*, WU Xiao-qin1, TANG Jian2   

  1. 1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. Anhui Forestry Department, Hefei 230031, China
  • Online:2007-08-18 Published:2007-08-18

摘要: <正>研究了南方主要绿化树种对重金属的积累特性,结果表明:同一树种中Pb、Cd、Hg、As的含量差异较大,相同元素在不同树种中的含量也存在一定差异,不同树种的重金属含量一般随环境污染程度的增加而升高,即从高至低依次为市区、近郊、远郊,市区树种中Pb、Cd、Hg、As的平均含量分别比远郊的增加62%、103%、187%、104%。垂枝榕Ficus beniamina‘Exotica’、菩提树F. religiosa、凤凰木Delonix regia、南洋杉Araucaria cunningghamii分别对Pb、Cd、Hg、As的积累作用较大,宜用这些树木进行重金属污染的治理和修复,洋紫荆Bauhinia variegata、南洋杉、高山榕F. altissima、小叶榕F. microcarpa分别对Pb、Cd、Hg、As的抗性最小、即敏感性最强,可用于重金属污染的监测。

Abstract: Bursaphelenchus xylophilus, B. mucronatus and the other nematodes separated from dead pine. were detected by using primer K09F2/R. The results indicated that the PCR product of all B. xylophilus isolates had a clear and bright fragment about 340bp. But B. mucronatus and other nematodes had not any fragments. So pairwise K09 F2/R was used for constructing identification kit of B. xylophilus. It took a short time to identify nematodes by adopting this kit. The time of total detection process was within two and half hours. Besides, the sensitivity of this kit reached to 1/7 of a nematode. The detection result is easy to estimate, and the cost of detection is quite low. This kit is a rapid, reliable and convenient means for detecting B. xylophilus.

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