<正>利用AFLP技术，对65个菊花品种进行了遗传多样性分析。选用6对多态性高、分辨力强的引物组合分别对供试材料的基因组DNA进行扩增，共获得244条清晰可辨的谱带，其中多态性带178条，多态位点百分率达72.95％，揭示了菊花栽培种质丰富的遗传多样性。对AFLP扩增结果采用UPGMA法进行聚类分析，多数瓣型相同的菊花种质表现出较为密切的亲缘关系，地域来源与聚类结果也有一定程度的相关性，而花色与聚类结果无明显的相关性。

The adsorptive behavior of Pb2+ and Cu2+ by humus was investigated. The result showed that the humus had a high adsorptive ability for Pb2+ and Cu2+ but there were significant difference. The humus has a much higher adsorptive ability on Pb2+ than on Cu2+. The adsorption isotherms of Cu2+ conform to Langmuir-equation was better than Freundlich-equation, but of Pb2+ both was well. According to the langmuir model, the maximum adsorption amount of Pb2+ and Cu2+ was 84.15mg/g and 13.09mg/g respectively. The former was 6.4 times of the latter. The pH value was the main factor affecting adsorption, humus at higher pH had a larger adsorption ability. The analysis of IR spectrum indicated that adsorption of Pb2+ and Cu2+ by humus caused the combination of carboxyl groups and hydroxy with heavy metal.