美国月桂樱的组织培养及植株再生

宿静,汤庚国*,王光萍,刘雅帅

南京林业大学学报(自然科学版) ›› 2008, Vol. 32 ›› Issue (04) : 98-100.

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南京林业大学学报(自然科学版) ›› 2008, Vol. 32 ›› Issue (04) : 98-100. DOI: 10.3969/j.jssn.1000-2006.2008.04.022
研究论文

美国月桂樱的组织培养及植株再生

  • 宿静,汤庚国*,王光萍,刘雅帅
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Tissue culture and plant regeneration of Laurocerasus caroliniana Ait.

  • SU Jing, TANG Geng-guo *, WANG Guang-ping, LIU Ya-shuai
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摘要

<正>以油脂下脚料酸化油为原料,通过与顺丁烯二酸酐加成,再与二甘醇酯化,得到聚酯多元醇,其最佳工艺条件为:M(酸化油):M(顺丁烯二酸酐):M(二甘醇)=1:1:3.3;顺丁烯二酸酐在180℃、195~200%:、200~220℃:分3次加入,备反应1h,在230~240℃下反应0.5h;240%:下常压酯化10h;240℃减压酯化1h。合成的聚酯多元醇酶值小于1mg/g。羟值在250~300mg/g。以合成的聚酯多元醇为原料,制备的双组分聚氨酯胶粘剂性能可以达到彩钢板与聚苯乙烯泡沫塑料的胶合工艺要求。

Abstract

The fasciculated shoots were induced by the explant achieved from the juvenile branches of Laurocerasus caroliniana Air. in spring. The inducing rate of fasciculated shoots attained 100%when they were cultured on the inducing medium of MS with BA O. 5 mg/L and NAA 0. O1 mg/L. The adventitious shoots were propagated most rapidly when subcuhured on the medium of MS with BA 1.0 mg/L and NAA 0.1 mg/L. Moreover,the adventitious shoots with best quality were obtained when they were cultured in the medium of MS with KT I. 0 mg/L and NAA 0. 1 mg/L. The results of this experiment also indicated that gibberellin had no effects on the elongation of adventitious shoots. When the 3--4 cm adventitious shoots were cut and rooted in the rooting medium of 1/2 MS with NAA 1. 0 mg/L,the rooting rate and surviving rate attained 100%and 40%respectively.

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宿静,汤庚国*,王光萍,刘雅帅. 美国月桂樱的组织培养及植株再生[J]. 南京林业大学学报(自然科学版). 2008, 32(04): 98-100 https://doi.org/10.3969/j.jssn.1000-2006.2008.04.022
SU Jing, TANG Geng-guo *, WANG Guang-ping, LIU Ya-shuai. Tissue culture and plant regeneration of Laurocerasus caroliniana Ait.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2008, 32(04): 98-100 https://doi.org/10.3969/j.jssn.1000-2006.2008.04.022
中图分类号: S722.3   

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