The fasciculated shoots were induced by the explant achieved from the juvenile branches of Laurocerasus caroliniana Air. in spring. The inducing rate of fasciculated shoots attained 100%when they were cultured on the inducing medium of MS with BA O. 5 mg/L and NAA 0. O1 mg/L. The adventitious shoots were propagated most rapidly when subcuhured on the medium of MS with BA 1.0 mg/L and NAA 0.1 mg/L. Moreover,the adventitious shoots with best quality were obtained when they were cultured in the medium of MS with KT I. 0 mg/L and NAA 0. 1 mg/L. The results of this experiment also indicated that gibberellin had no effects on the elongation of adventitious shoots. When the 3--4 cm adventitious shoots were cut and rooted in the rooting medium of 1/2 MS with NAA 1. 0 mg/L,the rooting rate and surviving rate attained 100%and 40%respectively.
SU Jing, TANG Geng-guo *, WANG Guang-ping, LIU Ya-shuai.
Tissue culture and plant regeneration of Laurocerasus caroliniana Ait.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2008, 32(04): 98-100 https://doi.org/10.3969/j.jssn.1000-2006.2008.04.022