杨四瘿螨诱导的美洲黑杨抑制消减文库

张萍; 潘惠新; 黄敏仁; 王明庥

doi:10.3969/j.jssn.1000-2006.2009.02.002

杨四瘿螨诱导的美洲黑杨抑制消减文库

张萍，潘惠新，黄敏仁*，王明庥

南京林业大学学报（自然科学版） ›› 2009, Vol. 33 ›› Issue (02) : 5.

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南京林业大学学报（自然科学版） ›› 2009, Vol. 33 ›› Issue (02) : 5. DOI: 10.3969/j.jssn.1000-2006.2009.02.002

研究论文

杨四瘿螨诱导的美洲黑杨抑制消减文库

张萍，潘惠新，黄敏仁*，王明庥

作者信息 +

Construction and analysis of Populus deltoides SSH cDNA library induced by Tetra lobulifera (Keifer)

ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu

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摘要

采用抑制性消减杂交(suppression subtractive hybridization，SSH)技术，以杨四瘿螨诱导48、72 h的美洲黑杨无性系为实验方(tester)，以未经诱导的美洲黑杨无性系为驱动方(driver)构建杨四瘿螨诱导差异表达的抑制消减cDNA文库，共获得了710个克隆，所建cDNA文库的插入片段主要集中在200~1 000 bp之间。通过序列测定拼接和同源性对比分析，获得了美洲黑杨涉及信号传递、能量代谢、胁迫响应、物质运输等功能的差异基因。

Abstract

A suppression subtractive hybridization (SSH) cDNA library was constructed with Populus deltoides induced by Tetra lobulifera (Keifer), the leaf was induced for 48 and 72 hours and used as the tester, whereas the induced leaf was employed as driver. Seven hundreds and ten cDNA clones were identified. PCR examination showed that most of the inserted fragments were 200—1 000 bp. The BlastN homology analysis revealed that these genes played varies roles in signal transduction, energy metabolism, response to stress and transportation etc.

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张萍，潘惠新，黄敏仁*，王明庥. 杨四瘿螨诱导的美洲黑杨抑制消减文库[J]. 南京林业大学学报（自然科学版）. 2009, 33(02): 5 https://doi.org/10.3969/j.jssn.1000-2006.2009.02.002

ZHANG Ping, PAN Huixin, HUANG Minren*, WANG Mingxiu. Construction and analysis of Populus deltoides SSH cDNA library induced by Tetra lobulifera (Keifer)[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2009, 33(02): 5 https://doi.org/10.3969/j.jssn.1000-2006.2009.02.002

中图分类号： S722

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基金

收稿日期：2008-04-07修回日期：2008-11-12基金项目：国家“十一五”科技支撑计划(2006BAD01A1503)；国家高技术研究发展计划(2006AA100109)作者简介：张萍(1980—)，博士生。*黄敏仁(通讯作者)，教授，研究方向为林木遗传育种。Email:mrhung@njfu.com.cn引文格式：张萍,潘惠新,黄敏仁,等. 杨四瘿螨诱导的美洲黑杨抑制消减文库的构建及分析[J]. 南京林业大学学报:自然科学版,2009,33(2):5-8.