从杨树叶片的双向电泳蛋白图谱上随机选取12个蛋白点，经电喷雾串联质谱(Electrospray ionization tandem mass spectrometry ，ESIMSMS)分析。结果显示，这12个蛋白点代表了5种不同的蛋白，其中包括：核酮糖二磷酸缩化酶/加氧酶(RuBisCO)、富甘氨酸RNA结合蛋白(Glycinerich RNAbinding protein)、微管蛋白(alphatubulin)、叶绿体磷酸甘油酸激酶(chloroplast phosphoglycerate kinase)以及光敏色素(phytochrome B2)蛋白等。
Abstract
Twelve protein spots were randomly selected from poplar leaf protein profiles of twodimension gel electrophoresis. By ESIMSMS analysis, the results showed that they represented five different proteins, including RuBisCO, Glycinerich RNAbinding protein, alphatubulin, chloroplast phosphoglycerate kinase and phytochrome B2. This study would lay the foundation for analysis of the whole proteome of poplar leaf and for the other biological research.
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参考文献
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基金
收稿日期：2008-04-05修回日期：2008-09-09基金项目：国家“十一五”科技支撑计划(2006BAD01A1503)作者简介：袁坤(1979—)，助理研究员，博士。*黄敏仁(通讯作者)，教授，研究方向为林木遗传育种。Email: mrhung@njfu.edu.cn。引文格式：袁坤,潘惠新,杨礼富,等. 杨树叶片蛋白质组分析[J]. 南京林业大学学报:自然科学版,2009,33(3):13-16.
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