南京林业大学学报(自然科学版) ›› 2009, Vol. 33 ›› Issue (03): 13-16.doi: 10.3969/j.jssn.1000-2006.2009.03.004

• 研究论文 • 上一篇    下一篇

杨树叶片蛋白质组分析

袁坤1,潘惠新2,杨礼富1,王真辉1,王明庥2,黄敏仁2*   

  1. 1.中国热带农业科学院橡胶研究所,海南儋州571737;2.南京林业大学杨树研究开发中心,江苏南京210037
  • 出版日期:2009-06-18 发布日期:2009-06-18
  • 基金资助:
    收稿日期:2008-04-05修回日期:2008-09-09基金项目:国家“十一五”科技支撑计划(2006BAD01A1503)作者简介:袁坤(1979—),助理研究员,博士。*黄敏仁(通讯作者),教授,研究方向为林木遗传育种。Email: mrhung@njfu.edu.cn。引文格式:袁坤,潘惠新,杨礼富,等. 杨树叶片蛋白质组分析[J]. 南京林业大学学报:自然科学版,2009,33(3):13-16.

The preliminary analysis of poplar leaf proteome

YUAN Kun1, PAN Huixin2, YANG Lifu1, WANG Zhenhui1, WANG Mingxiu2, HUANG Minren2*   

  1. 1.Rubber Research Institute Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China; 2.Research Center of Polar, Nanjing Forestry University, Nanjing 210037, China
  • Online:2009-06-18 Published:2009-06-18

摘要: 从杨树叶片的双向电泳蛋白图谱上随机选取12个蛋白点,经电喷雾串联质谱(Electrospray ionization tandem mass spectrometry ,ESIMSMS)分析。结果显示,这12个蛋白点代表了5种不同的蛋白,其中包括:核酮糖二磷酸缩化酶/加氧酶(RuBisCO)、富甘氨酸RNA结合蛋白(Glycinerich RNAbinding protein)、微管蛋白(alphatubulin)、叶绿体磷酸甘油酸激酶(chloroplast phosphoglycerate kinase)以及光敏色素(phytochrome B2)蛋白等。

Abstract: Twelve protein spots were randomly selected from poplar leaf protein profiles of twodimension gel electrophoresis. By ESIMSMS analysis, the results showed that they represented five different proteins, including RuBisCO, Glycinerich RNAbinding protein, alphatubulin, chloroplast phosphoglycerate kinase and phytochrome B2. This study would lay the foundation for analysis of the whole proteome of poplar leaf and for the other biological research.

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