Abstract: A study was conducted on responses of some physiological and biochemical indices, such as leaf electrolyte leakage of cell membrane, soluble sugars, free praline, POD and polyamine in five evergreen broadleaved tree species introduced from other provinces and cultivated in Nanjing, to variations of low temperature during winter season. The main results were described as follows: (1) The trend was represented that the electrolyte leakage of leaf cell membrane of all five tree species except Parakmeria lotungensis increased at the beginning, then decreased and increased finally, that of Cinnamomum chekiangense was relatively steady, and its changing extent in leaf of Manglietia insignis was the largest. (2) Leaf soluble sugar contents, free proline contents , POD activity, Put contents, Spd contents and Spm contents of five tree species increased first and then decreased. (3) Leaf soluble sugar contents of five tree species were adjusted to the highest value before the lowest temperature occured. Leaf free proline contents of five tree species accumulated gradually with longer lowtemperature time and stronger low temperature intensity. There was a highest variation range in leaf free proline of both Elaeocarpus glabripetalus Merr and Parakmeria lotungensis. Leaf POD activity peak value of Parakmeria lotungensis appeared in early months and kept steady. That of Cinnamomum chekiangense was higher and kept steady, but those of Manglietia insignis and Michelia chapensis Dandy were unstable. (4) Monthly LT50 value of Cinnamomum chekiangense and Parakmeria lotungensis was lower than those of other species. There was a significant correlation between LT50 value of five tree species except Cinnamomum chekiangense and the mean daily lowest temperature of ten days before sampling. (5) All five tree species except Michelia chapensis Dandy regulated their leaf contents of polyamine in adaptation to low air temperature changes.