研究了以酶法制备和以高温降解制备的低聚木糖体外对青春双歧杆菌的增殖作用以及双歧杆菌代谢低聚木糖的规律。结果表明：以酶法制备的3 g/L低聚木糖为碳源时，菌体浓度在18 h达到最大值0.43 g/L，增殖了3.73倍；以高温降解的3 g/L低聚木糖为碳源时，菌体的浓度24 h时达到最大值0.19 g/L，增殖1.16倍。酶法制备低聚木糖增殖效果优于高温降解低聚木糖。在代谢过程中发现青春双歧杆菌优先利用木二、木三糖，在利用聚合度3以上的低聚木糖的同时会释放出阿拉伯糖和木糖。青春双歧杆菌对木二、木三、木四、木五和木六糖利用率分别为9400 %、50.00 %、83.87 %、60.87 %和48.28 %，其代谢产物主要是乳酸、乙酸和丙酸。
Abstract
The proliferation of xylooligosaccharides (XOS) produced from enzyme hydrolysis and hightemperature degradation of xylan to Bifidobacterium adolescentis in vitro was studied. The results showed that in vitro fermentation of B.adolescentis employed 3 g/L enzymatic prepared XOS as carbon sources for 18 h, the highest cell concentration reached 0.43 g/L, and cell proliferation was more than 3.73 times than that of the initial. When B.adolescentis incubation used 3 g/L XOS which prepared from hightemperature degradation of xylan as carbon sources, the maximal cell concentration of 0.19 g/L was obtained for 24 h fermentation and 1.16 times proliferation than that of the initial. It was found that B.adolescentis could metabolize xylobiose and xylotriose preferentially, and released arabinose and xylose while consuming the XOS that DP above 3. It was shown that xylobiose, xylotriose, xyloteraose, xylopentaose, xylohexaose were consumed 94.00 %, 50.00 %, 83.87 %, 60.87 % and 48.28 % of the initial by B.adolescentis. The mainly metabolites of B.adolescentis to XOS were lactate, acetate and propionate.
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基金
收稿日期：2009-01-18修回日期：2009-09-03基金项目：国家林业局林业公益性行业科研专项(200904017)作者简介：张晓萍(1980—)，博士生。*余世袁(通信作者)，教授。Email: syu@njfu.edu.cn。引文格式：张晓萍,勇强,余世袁. 青春双歧杆菌体外代谢低聚木糖的研究[J]. 南京林业大学学报:自然科学版,2010,34(1):5-8.
