刚毛柽柳TheIF1A基因转入酵母的抗逆能力分析

doi:10.3969/j.issn.1000-2006.2014.05.013

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (05): 62-66.doi: 10.3969/j.issn.1000-2006.2014.05.013

刚毛柽柳TheIF1A基因转入酵母的抗逆能力分析

杨桂燕,于丽丽,赵玉琳,赵震,高彩球^*

东北林业大学,林木遗传育种国家重点实验室,黑龙江哈尔滨 150040

出版日期:2014-10-31 发布日期:2014-10-31
基金资助:
收稿日期:2013-11-13 修回日期:2014-03-25
基金项目:国家自然科学基金项目(31370676)
第一作者:杨桂燕,博士生。*通信作者:高彩球,副教授。E-mail:gaocaiqiu@nefu.edu.cn。
引文格式:杨桂燕,于丽丽,赵玉琳,等. 刚毛柽柳TheIF1A基因转入酵母的抗逆能力分析[J]. 南京林业大学学报:自然科学版,2014,38(5):62-66.

Stress tolerance analysis of a Tamarix hispida TheIF1A in Saccharomyces cerevisiae

YANG Guiyan, YU Lili, ZHAO Yulin, ZHAO Zhen, GAO Caiqiu^*

State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China

Online:2014-10-31 Published:2014-10-31

摘要/Abstract

摘要： 翻译起始因子eIF1A基因是蛋白合成的重要调控因子之一,在一些植物中可能参与抗逆调控过程。为了研究刚毛柽柳TheIF1A基因是否参与抗逆过程,将TheIF1A基因插入酵母表达载体pYES2中构建成重组载体,转入酿酒酵母(Saccharomyces cerevisiae)中获得重组型酵母,分别比较转TheIF1A基因酵母和转空载体对照酵母在山梨醇、H₂O₂、CdCl₂、CuSO₄、ZnCl₂、KCl、Na₂CO₃、MgCl₂、-20 ℃和53 ℃胁迫处理之后的存活能力。结果显示,TheIF1A基因能有效提高转基因酵母的抗干旱、盐碱、氧化、重金属及极端温度的能力,表明TheIF1A可能参与了柽柳多种抗逆调控过程。

Abstract: Translation initiation factor 1A is an important regulation factor for protein synthesis, which may involve in plant abiotic stress regulation. In order to study the stress tolerance of a translation initiation factor 1A(TheIF1A) from Tamarix hispida, the recombinant plasmid, named as pYES2-TheIF1A, was constructed by inserting the TheIF1A gene into the yeast expression vector pYES2. The plasmid pYES2-TheIF1A was transformed into the yeast Saccharomyces cerevisiae, and the yeast transformed with empty pYES2 was employed as a control. The pYES2-TheIF1A and pYES2 recombinant yeast were respectively treated with sorbitol, H₂O₂, CdCl₂, CuSO₄, ZnCl₂, KCl, Na₂CO₃, MgCl₂, low temperature -20 ℃ and high temperature 53 ℃, respectively. Results showed that the yeast expressing TheIF1A had greater tolerant to salt, drought, heavy metals, and both low and high temperatures than the control, suggesting that TheIF1A gene might an important stress-regulating gene in T.hispida.

中图分类号:

Q943.2

杨桂燕,于丽丽,赵玉琳,等. 刚毛柽柳TheIF1A基因转入酵母的抗逆能力分析[J]. 南京林业大学学报（自然科学版）, 2014, 38(05): 62-66.

YANG Guiyan, YU Lili, ZHAO Yulin, ZHAO Zhen, GAO Caiqiu. Stress tolerance analysis of a Tamarix hispida TheIF1A in Saccharomyces cerevisiae[J].Journal of Nanjing Forestry University (Natural Science Edition), 2014, 38(05): 62-66.DOI: 10.3969/j.issn.1000-2006.2014.05.013.

参考文献

[1] Howarth C. Molecular responses of plants to an increased incidence of heat shock[J]. Plant, Cell & Environment, 1991, 14(8): 831-841.
[2] Dong Z, Zhang J. Initiation factor eIF3 and regulation of mRNA translation, cell growth, and cancer[J]. Critical Reviews in Oncology/Hematology, 2006, 59(3): 169-180.
[3] Pyronnet S, Sonenberg N. Cell-cycle-dependent translational control[J]. Current Opinion in Genetics & Development, 2001, 11(1): 13-18.
[4] Groul T, Ivanov P, FrydlováI, et al. Robust heat shock induces eIF2α-phosphorylation-independent assembly of stress granules containing eIF3 and 40 s ribosomal subunits in budding yeast, Saccharomyces cerevisiae[J]. Journal of Cell Science, 2009, 122(12): 2078-2088.
[5] Singh G, Jain M, Kulshreshtha R, et al. Expression analysis of genes encoding translation initiation factor 3 subunit G(TaeIF3G)and vesicle-associated membrane protein-associated protein(TaVAP)in grought tolerant and susceptible cultivars of wheat[J]. Plant Science, 2007, 173(6): 660-669.
[6] Rangan L, Rout A, Sudarshan M, et al. Molecular cloning, expression and mapping of the translational initiation factor eIF1 gene in Oryza sativa[J]. Functional Plant Biology, 2009, 36(5): 442-452.
[7] Wang L, Xu C, Wang C, et al. Characterization of a eukaryotic translation initiation factor 5A homolog from Tamarix androssowii involved in plant abiotic stress tolerance[J]. BMC Plant Biology, 2012, 12(1): 1471-2229.
[8] 张道远, 张娟, 谭敦炎, 等. 国产柽柳科3属6种植物营养枝的解剖观察[J]. 西北植物学报, 2003, 23(3): 382-388.Zhang D Y, Zhang J, Tan D Y, et al. Anatomical observation of young branches of 6 species of Tamaricaceae from China[J].Acta Bot Boreal-Occident Sin, 2003, 23(3): 382-388.
[9] 尹林克. 中亚荒漠生态系统中的关键种——柽柳(Tamarix spp.)[J]. 干旱区研究, 1995, 12(3): 43-47. Yin L K. Tamarix spp.—The keystone species of desert ecosystem[J]. Arid Zone Reasearch, 1995, 12(3): 43-47.
[10] Yang J, Wang Y, Liu G, et al. Tamarix hispida metallothionein-like ThMT3, a reactive oxygen species scavenger, increases tolerance against Cd²⁺, Zn²⁺, Cu²⁺, and NaCl in transgenic yeast[J]. Molecular Biology Reports, 2011, 38(3): 1567-1574.
[11] Gao C, Jiang B, Wang Y, et al. Overexpression of a heat shock protein(ThHSP18.3)from Tamarix hispida confers stress tolerance to yeast[J]. Molecular Biology Reports, 2012, 39(4): 4889-4897.
[12] Wang B, Wang Y, Zhang D, et al. Verification of the resistance of a LEA gene from Tamarix expression in Saccharomyces cerevisiae to abiotic stresses[J]. Journal of Forestry Research, 2008, 19(1): 58-62.
[13] Gao C, Wang Y, Jiang B, et al. A novel vacuolar membrane H⁺-ATPase c subunit gene(ThVHAc1)from Tamarix hispida confers tolerance to several abiotic stresses in Saccharomyces cerevisiae[J]. Molecular Biology Reports, 2011, 38(2): 957-963.
[14] 张凯敏, 王玉成, 杨桂燕, 等. 柽柳ThPR1基因的克隆与表达分析[J]. 南京林业大学学报:自然科学版, 2013, 37(2): 45-49. Zhang K M, Wang Y C, Yang G Y, et al.Clone and expression analysis of ThPR1 gene in Tamarix hispida[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2013, 37(2):45-49.
[15] 闫绍鹏, 杨瑞华, 王秋玉. 低温胁迫对欧美杂种山杨无性系的生理影响[J]. 南京林业大学学报:自然科学版, 2013, 37(6): 161-164. Yan S P, Yang R H, Wang Q Y.Physiological influence of low temperature stress on hybrid clones of Populus tremula×P. tremuloides[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2013, 37(6): 161-164.
[16] Ptushkina M, Malys N, McCarthy J E. eIF4e isoform 2 in Schizosaccharomyces pombe is a novel stress-response factor[J]. EMBO Reports, 2004, 5(3): 311-316.
[17] Rausell A, Kanhonou R, Yenush L, et al. The translation initiation factor eIF1A is an important determinant in the tolerance to NaCl stress in yeast and plants[J]. The Plant Journal, 2003, 34(3): 257-267.
[18] Latha R, Salekdeh G H, Bennett J, et al. Molecular analysis of a stress-induced cDNA encoding the translation initiation factor, eIF1, from the salt-tolerant wild relative of rice, Porteresia coarctata[J]. Functional Plant Biology, 2004, 31(10): 1035-1042.

相关文章 13

[1]	高源, 孙佳彤, 周晨光, 姜立泉, 李伟, 李爽. LBD12转录因子调控毛果杨木材形成研究[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 29-38.
[2]	贾展慧, 贾晓东, 许梦洋, 莫正海, 翟敏, 宣继萍, 张计育, 王刚, 王涛, 郭忠仁. 薄壳山核桃原花青素合成关键酶基因的克隆与表达分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 49-57.
[3]	王竹雯, 国艳娇, 李爽, 周晨光, 姜立泉, 李伟. 基于CRISPR/Cas9的毛果杨PtrHBI1基因功能解析[J]. 南京林业大学学报（自然科学版）, 2021, 45(6): 31-39.
[4]	侯静, 毛金燕, 翟惠, 王洁, 尹佟明. CRISPR/Cas技术在木本植物改良中的应用[J]. 南京林业大学学报（自然科学版）, 2021, 45(6): 24-30.
[5]	孙佳彤, 国艳娇, 李爽, 周晨光, 姜立泉, 李伟. 基于CRISPR/Cas9的毛果杨bHLH106转录因子的功能研究[J]. 南京林业大学学报（自然科学版）, 2021, 45(6): 15-23.
[6]	施季森. CRISPR:从“盲盒”基因编辑到“精准靶向”基因组编辑的未竟之旅[J]. 南京林业大学学报（自然科学版）, 2021, 45(6): 12-14.
[7]	王培龙, 杨妮, 张傲然, 唐努尔·塞力克, 李爽, 高彩球. 刚毛柽柳ThPCS1基因克隆与镉胁迫应答分析[J]. 南京林业大学学报（自然科学版）, 2021, 45(3): 71-78.
[8]	莫正海, 李风达, 苏文川, 曹凡, 彭方仁, 李永荣. 薄壳山核桃嫁接愈合过程中CiMYB46基因的克隆与表达分析[J]. 南京林业大学学报（自然科学版）, 2019, 43(5): 156-162.
[9]	刘晓威,杨秀艳,武海雯,刘肖艳,朱建峰,张华新. NaCl胁迫下红砂种子萌动期差异表达基因的转录组分析[J]. 南京林业大学学报（自然科学版）, 2019, 43(03): 28-36.
[10]	刘中原,姜波,吕佳欣,李新苹,高彩球. 刚毛柽柳Th2CysPrx基因的互作蛋白及其表达模式分析[J]. 南京林业大学学报（自然科学版）, 2019, 43(02): 86-92.
[11]	田晶,赵雪媛,谢隆聖,权晋谊,姚连梅,王国东,郑要强,刘雪梅. SPL转录因子调控植物花发育及其分子机制研究进展[J]. 南京林业大学学报（自然科学版）, 2018, 42(03): 159-166.
[12]	官民晓,刘雪梅,张妍,刘瀛,孙丰宾. 白桦SPL8转录因子基因的分离及转录表达分析[J]. 南京林业大学学报（自然科学版）, 2013, 37(03): 17-22.
[13]	张凯敏,王玉成,杨桂燕,高彩球. 柽柳ThPR1基因的克隆与表达分析[J]. 南京林业大学学报（自然科学版）, 2013, 37(02): 45-49.

刚毛柽柳TheIF1A基因转入酵母的抗逆能力分析

Stress tolerance analysis of a Tamarix hispida TheIF1A in Saccharomyces cerevisiae

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 13

编辑推荐

Metrics

本文评价

作者

读者

审稿