在温室内以长白落叶松幼苗为材料进行砂培试验,探讨了4种不同氮素浓度(1、4、8、16 mmol/L代号分别为N1、N4、N8、N16)处理对长白落叶松幼苗生物量以及根、茎、叶氮磷分配的影响。结果表明:①氮素供给浓度显著影响幼苗生物量,7、8、9月份均在N8水平下达最大,从7月份到9月份,叶片生物量占全株比例逐渐降低,而根系生物量所占比例逐渐增加。②随着供氮水平的提高,苗木根、茎、叶中氮浓度明显增加。生长末期根、茎、叶中氮浓度平均达到生长初期的1.46、1.48、1.17倍。不同氮处理下幼苗根、茎和叶全磷浓度呈现波动性变化。③幼苗体内氮贮量随着落叶松的生长呈明显上升趋势。9月份全株氮贮量比7月份平均增加了2.86倍。氮贮量分配在不同部位有很大不同,分配到叶片中的比例在7月份最高,平均为60%,而分配到幼苗根系中氮贮量的比例随苗木的生长而逐渐增加,在9月份相对值最高,达到41%。不同氮处理之间氮贮量分配比例相差不大。④长白落叶松幼苗根、茎、叶磷贮量变化规律与氮贮量一致,只是变化幅度低于氮。
Abstract
The effects of different concentration of nitrogen treatments on the biomass, nitrogen and phosphorus partition in roots, stems and leaves of one-year-old Larix olgensis seedlings were studied by a sand culture experiment in greenhouse. The results suggested that N supply could significantly increase the seedling biomass. The biomass of roots, stems and leaves were the highest when 8 mmol/L nitrogen was applied in July, August and September. The biomass of leaves decreased gradually, while that of roots increased from July to September. The N concentrations increased significantly along with the N supply levels increasing. The mean N concentrations in roots, stems and leaves in the latter growth stage were 1.46, 1.48 and 1.17 times respectively more than that in the initial stage. The P concentrations fluctuated with the N levels change. The N stocks of whole plant increased markedly as the growth of plant, and it was 2.86 times more in September than in July. The N allocation also varied in leaf, stem, and root. The proportion of N allocation in leaf was the highest in July(60% in average). The proportion of N allocation in root increased with plant growth, which was the highest in September(41% in average). There was no obvious difference in N stocks among different N levels. The P stocks of the roots, stems and leaves were basically consistent with the N, but the variation scope was smaller than that of N.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] 赵平,孙谷畴,彭少麟.植物氮素营养的生理生态学研究[J].生态科学, 1998,17(2):37-42.Zhao P,Sun G C, Peng S L. Ecophysiological research on nitrogen nutrition of plant[J].Ecologic Science, 1998, 17(2):37-42.
[2] Cassman K G, Kropff M J, Gaunt J, et al. Nitrogen use efficiency of rice reconsidered: what are the key constraints?[J]. Plant and Soil, 1993, 155:359-362.
[3] Crawford N M, Glass A D M. Molecular and physiological aspects of nitrate uptake in plants[J]. Trends in Plant Science, 1998,3(10):389-395.
[4] 祝燕,李国雷,李庆梅,等.持续供氮对长白落叶松播种苗生长及抗寒性的影响[J].南京林业大学学报:自然科学版,2013,37(1):44-48.Zhu Y, Li G L, Li Q M, et al. Influence of continually supplementing nitrogen on the quality of Larix olgensis seedlings[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2013, 37(1):44-48.
[5] 吉艳芝,陈立新,冯万忠.施肥对落叶松人工林的生长量和光合特性的影响[J].河北农业大学学报,2004,27(3):45-48.Ji Y Z, Chen L X, Feng W Z. Effects of fertilization on growth amount and photosynthetic characteristics in larch plantations[J]. Journal of Agricultural University of Hebei, 2004, 32(1):18-21.
[6] 周利勋,刘广平.施肥对红松和落叶松幼苗各器官养分含量的影响[J].东北林业大学学报,2004,32(4)18-21.Zhou L X, Liu G P. Effect of fertilization on nutrient concentration of organs for Korean pine and larch seedlings[J]. Journal of Northeast Forestry University, 2004, 27(3):45-48.
[7] Jarkko U, Toini H. Influence of nitrogen and phosphorus availability and ozone stress on Norway spruce seedlings[J]. Tree Physiol, 2001, 21:447-456.
[8] 李海霞,李正华,郭树平,等.不同氮磷水平对红松幼苗碳氮积累与分配的影响[J].西北林学院学报,2013,28(5):24-29.Li H X,Li Z H,Guo S P, et al. Effects of different nitrogen and phosphorus supply levels on C, N accumulation and partitioning in Pinus koraiesis seedlings[J].Journal of Northwest Forestry University,2013,28(5):24-29.
[9] 李海霞,李正华,戴伟男,等.氮磷水平对中美山杨幼苗碳氮积累与分配的影响[J].西南林业大学学报,2013,33(3):8-14.Li H X,Li Z H,Dai W N, et al. Effect of N and P supply levels on C, N accumulation and distribution in Populus davidiana×P. tremuloides seedlings[J]. Journal of Southwest Forestry University,2013,33(3):8-14.
[10] 中国林业科学研究院林业研究所.LY/T 1232—1999 森林土壤全磷的测定[S].北京:中国标准出版社,1999.
[11] Hendricks J J, Nadelhoffer K J, Aber J D. Assessing the role of fine roots in carbon and nutrient cycling[J]. Trends in Ecology & Evolution, 1993, 8(5):174-178.
[12] Ludovici K H, Morris L A. Response of loblolly pine, sweetgum and crab grass roots to localized increases in nitrogen in two watering regimes[J]. Tree Physiology, 1996, 16:933-939.
[13] Lu X K, Mo J M, Gundersern P, et al. Effect of simulated N deposition on soil exchangeable cations in three forest types of subtropical China[J]. Pedosphere, 2009, 19(2): 189-198.
[14] Miller A J,Fan X R, Orsel M, et al. Nitrate transport and signaling[J]. Journal of Experimental Botany, 2007, 58(9): 2297-2306.
[15] Jones H E, Högberg P, Ohlsson H. Nutrient assessment of a forest fertilization experiment in northern Sweden by root bioassays[J]. Forest Ecology and Management, 1991, 64(1):59-69.
[16] 范志强.氮磷营养及氮形态对水曲柳幼苗生长和生理的影响机制[D].哈尔滨:东北林业大学,2004.Fan Z Q. Effects of nitrogen, phosphorus and nitrogen forms on growth and physiological traits of Fraxinus mandshurica seedings[D].Harbin: Northeast Forestry University,2004.
[17] 沈善敏,宇万太,张璐,等.杨树主要营养元素内循环及外循环研究.Ⅰ:落叶前后各部位养分浓度及养分贮量变化[J].应用生态学报,1992,3(4):296-301.Shen S M, Yu W T, Zhang L, et al. Internal and external nutrient cyclings of poplar tree.Ⅰ:Changes of nutrient storage indifferent parts of poplar tree before and after leaf fallen[J]. Chinese Journal of Applied Ecology, 1992, 3(4):296-301.
[18] 沈善敏,宇万太,张璐,等.杨树主要营养元素内循环及外循环研究.Ⅱ:落叶前后各部位养分在植株体内外的迁移和循环[J].应用生态学报,1993,4(1):27-31.Shen S M, Yu W T, Zhang L, et al. Internal and external nutrient cyclings of poplar tree.Ⅱ:Transferring and cycling of nutrients in and out of the tree before and after leaf fallen[J]. Chinese Journal of Applied Ecology, 1993, 4(1):27-31.
[19] Carswell F E, Grace J, Lucas M E, et al. Interaction of nutrient limitation and elevated CO2 concentration on carbon assimilation of a tropical tree seedling(Cedrela odorata)[J].Tree Physiology, 2000, 20:977-986.
[20] Burton A J, Pregitzer K S, Hendrick R L. Relationships between fine root dynamics and nitrogen availability in Michigan northern hardwood forests[J].Oecologia, 2000, 125:389-399.
[21] Läuchli A, Bieleski R L.植物的无机营养[M].张礼中,毛知耘,译.北京:农业出版社,1992.
[22] Nakaji T, Fukami M, Dokiya Y, et al. Effects of high nitrogen load on growth, photosynthesis and nutrient status of Cryptomeria japonica and Pinus densiflora seedlings[J].Trees, 2001, 15:453-461.
[23] 范志强,王政权,吴楚,等.不同供氮水平对水曲柳苗木生物量、氮分配及其季节变化的影响[J].应用生态学报,2004,15(9):1498-1501.Fan Z Q, Wang Z Q, Wu C, et al. Effect of different nitrogen supply on Fraxinus mandshurica seedling’s biomass, N partitioning and their seasonal variation[J]. Chinese Journal of Applied Ecology, 2004, 15(9):1497-1501.
基金
收稿日期:2013-08-30 修回日期:2014-03-02
基金项目:黑龙江省科技攻关重大项目(GA09B202-02); 黑龙江省财政厅科研计划项目(2012-01)
第一作者:李海霞,助理研究员。*通信作者:白卉,副研究员。E-mail:baihui1979@163.com。郭树平,研究员。E-mail:hljgsp@126.com。
引文格式:李海霞,许传玲,郭树平,等. 供氮水平对长白落叶松幼苗生物量、氮磷浓度及其季节变化的影响[J]. 南京林业大学学报:自然科学版,2014,38(5):79-84.
PDF(1556482 KB)
