不同活性炭对杂交鹅掌楸体胚发生的影响

doi:10.3969/j.issn.1000-2006.2016.02.010

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (02): 59-64.doi: 10.3969/j.issn.1000-2006.2016.02.010

不同活性炭对杂交鹅掌楸体胚发生的影响

鲁路,陆叶,盛宇,郑晨,施季森,陈金慧^*

南京林业大学南方现代林业协同创新中心, 林木遗传与生物技术省部共建教育部重点试验室,江苏南京 210037

出版日期:2016-04-18 发布日期:2016-04-18
基金资助:
收稿日期:2015-07-20 修回日期:2015-11-26
基金项目:国家高技术研究发展计划(2013AA102705); 国家自然科学基金项目(31170619); 教育部新世纪优秀人才支持计划; 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:鲁路(736239523@qq.com)。*通信作者:陈金慧(chenjh@njfu.edu.cn),教授,博士。
引文格式:鲁路,陆叶,盛宇,等. 不同活性炭对杂交鹅掌楸体胚发生的影响[J]. 南京林业大学学报(自然科学版),2016,40(2):59-64.

Effects of different activated carbon on somatic embryogenesis of Liriodendron hybrids

LU Lu, LU Ye, SHENG Yu, ZHENG Chen, SHI Jisen, CHEN Jinhui^*

Co-Innovation Center for the Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

Online:2016-04-18 Published:2016-04-18

摘要/Abstract

摘要： 活性炭在体胚发生过程中能够促进细胞的生长及发育。为进一步提高杂交鹅掌楸体胚发生效率,以两个基因型的杂交鹅掌楸愈伤组织为材料,在培养过程中添加磷酸碳、针剂碳和Sigma公司活性炭,通过统计体胚总数及子叶胚数量,测定培养基的pH和渗透压,分析活性炭的吸附性能等,以筛选适合杂交鹅掌楸体胚发生的活性炭类型,并探索不同活性炭影响杂交鹅掌楸体胚发生效率差异的原因。研究结果表明:针剂碳、磷酸碳处理下诱导获得的杂交鹅掌楸体胚总数和子叶胚数显著高于Sigma公司活性炭,且针剂碳的效果最佳。针剂碳的吸附性能适中,添加针剂碳的培养基高压灭菌后其pH和渗透压也适中,说明活性炭的吸附性能会影响培养基的pH和渗透压,从而影响杂交鹅掌楸的体胚发生。

Abstract: Activated carbon is often used to improve cell growth and development in somatic embryogenesis. In this study, three types of activated carbon, including phosphoric acid activated carbon, activated carbon for refinement of injection and activated carbon purchased from Sigma were added respectively to the mediums for improving somatic embryogenesis frequency of two genotypes of cell lines in Liriodendron hybrids. The number of total somatic embryos and cotyledon embryos were counted, pH and osmotic pressure of the mediums were detected, and the adsorption ability of activated carbon were analyzed to select the most suitable activated carbonl and to find their potential effects on somatic embryogenesis of Liriodendron hybrids. The results showed that the first two types of activated carbon induced more total somatic embryos and cotyledon embryos than the third type, especially the activated carbon for refinement of injection. The adsorption ability of activated carbon for refinement of injection was moderate among the three, and the pH and osmotic pressure of the mediums containing it were also temperate compared with the other two types of activated carbon. It was concluded from the results that the adsorption ability of activated carbon may change the pH and osmotic pressure of mediums directly to influence the somatic embryogenesis of Liriodendron hybrids.

中图分类号:

S718

鲁路,陆叶,盛宇,等. 不同活性炭对杂交鹅掌楸体胚发生的影响[J]. 南京林业大学学报（自然科学版）, 2016, 40(02): 59-64.

LU Lu, LU Ye, SHENG Yu, ZHENG Chen, SHI Jisen, CHEN Jinhui. Effects of different activated carbon on somatic embryogenesis of Liriodendron hybrids[J].Journal of Nanjing Forestry University (Natural Science Edition), 2016, 40(02): 59-64.DOI: 10.3969/j.issn.1000-2006.2016.02.010.

参考文献

[1] 王红梅.活性炭在植物组织培养中的应用[J].上海农业科技, 2011(4):19.Doi:10.3969/j.issn.1001-0106.2011.04.013.
[2] 刘用生, 李友勇. 植物组织培养中活性炭的使用[J]. 植物生理学通讯, 1994, 30(3): 214-217. Liu Y S, Li Y Y. The use of activated charcoal in plant tissue culture[J]. Plant Physiology Journal, 1994, 30(3): 214-217.
[3] Monser L, Adhoum N. Modified activated carbon for the removal of copper, zinc, chromium and cyanide from wastewater [J]. Separation and Purification Technology, 2002, 26(2-3): 137-146. Doi:10.1016/S1383-5866(01)00155-1.
[4] Pullman G S, Bucalo K. Pine somatic embryogenesis: analyses of seed tissue and medium to improve protocol development [J]. New Forests, 2014, 45(3): 353-377.Doi:10.1007/s11056-014-9407-y.
[5] Ju H J, Jeyakumar J, Kamaraj M, et al. High frequency somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of gherkin(Cucumis anguria L.)[J]. Scientia Horti Culturae, 2014, 169: 161-168. Doi:10.1016/j.scienta.2014.02.023.
[6] Thuzar M, Vanavichit A, Tragoonrung S, et al. Efficient and rapid plant regeneration of oil palm Zygotic embryos cv. tenera through somatic embryogenesis [J]. Acta Physiologiae Plantarum, 2011, 33(1): 123-128.Doi:10.1007/s11738-010-0526-6.
[7] Manchanda P, Gosal S S. Effect ofactivated charcoal, carbon sources and gelling agents on direct somatic embryogenesis and regeneration in sugarcane via leaf roll segments [J]. Sugar Tech, 2012, 14(2): 168-173.Doi:10.1007/S12355-012-0143-3.
[8] Sáenz L, Herrera-Herrera G, Uicab-Ballote F, et al. Influence of form of activated charcoal on embryogenic callus formation in coconut(Cocos nucifera)[J]. Plant Cell, Tissue and Organ Culture(PCTOC), 2010, 100(3): 301-308.Doi:10.1007/s11240-009-9651-6.
[9] 李萍, 成仿云, 张颖星. 防褐剂对牡丹组培褐化发生, 组培苗生长和增殖的作用[J]. 北京林业大学学报, 2008, 30(2): 71-76.Doi:10.3321/j.issn:1000-1522.2008.02.012. Li P, Cheng F Y, Zhang Y X. Effects of browning antagonists on antibrowning, growth and multiplication of tissue culture of tree peony [J]. Journal of Beijing Forestry University, 2008, 30(2): 71-76.
[10] 肖颖, 王刚. 适于制作人工种子的一品红体细胞胚的培养[J]. 园艺学报, 2006, 33(1): 175-178.Doi:10.3321/j.issn:0513-353X.2006.01.041. Xiao Y, Wang G. Studies on high quality somatic embryogenesis applied to artificial seed of Euphorbia pulcherrima Willd.[J]. Acta Horticulturae Sinica, 2006, 33(1): 175-178.
[11] 郭成源.杂种鹅掌楸[J].农业知识, 2007, 32: 46. Guo C Y. Liriodendron hybrids [J]. Agricultural Knowledge, 2007, 32: 046.
[12] 陈金慧,施季森,诸葛强,等.杂交鹅掌楸的不定芽诱导及植株再生[J].植物生理学通讯, 2002, 38(5):459-459. Chen J H, Shi J S, Zhu G Q, et al. Adventitious bud induction and plantlet regeneration of Liriodendron hybrid(L.chinense×L.tulipifera)[J].Plant Physiology Communications, 2002, 38(5): 459-459.
[13] Merkle S A, Hoey M T, Watson-Pauley B A, et al. Propagation of Liriodendron hybrids via somatic embryogenesis[J]. Plant Cell, Tissue and Organ Culture, 1993, 34(2): 191-198.Doi:10.1007/BF00036101.
[14] 季孔庶. 杂交鹅掌楸的无性繁殖[J]. 南京林业大学学报(自然科学版), 2005,29(1):83-87.Doi:10.3969/j.issn.1000-2006.2005.01.020. Ji K S. Clonal propagation of Liriodendron chinense ×L. tulipifera[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2005,29(1):83-87.
[15] 陈金慧.杂交鹅掌楸体细胞胚胎发生研究[J].林业科学, 2003, 39(4): 49-53. Chen J H. Studies on the somatic embryogenesis of Liriodendron hybrids [J]. Scientia Silvae Sinicae, 2003, 39(4):49-53.
[16] 李婷婷,陈金慧,龙伟,等. 杂交鹅掌楸体胚发生悬浮系建立及同步化发育的调控研究[C]//第六届全国林木遗传育种大会论文集, 2008:2.
[17] 陈志, 陈金慧, 边黎明, 等. 杂交鹅掌楸胚性细胞悬浮系的建立[J]. 分子植物育种, 2007, 5(1): 137-140.Doi:10.3969/j.issn.1672-416X.2007.01.025. Chen Z, Chen J H, Bian L M, et al. Establishment of embryogenic callus suspension culture system in Liriodendron hybrids [J]. Molecular Plant Breeding, 2007, 5(1): 137-140.
[18] 施季森, 李婷婷, 陈金慧. 杂交鹅掌楸体胚发生同步化控制方法:CN102037896A[P]. 2010-09-28.
[19] 张会平,叶李艺,杨立春.氯化锌活化法制备木质活性炭研究[J]. 材料科学与工艺, 2006(1): 42-45.Doi:10.3969/j.issn.1005-0299.2006.01.013. Zhang H P, Ye L Y, Yang L C. Preparation of activated carbon from sawdust by chemical activation with zinc chloride [J]. Materials Science and Technology, 2006(1): 42-45.
[20] 林冠烽.磷酸法自成型木质颗粒活性炭的制备过程与机理研究[D].北京:中国林业科学研究院, 2013. Lin G F. Preparation of self-form granular activated carbon from lignocellulosic material by phosphoric acid activation and analysis of its self-from mechanism [D].Beijing:Chinese Academy of Forestry, 2013.
[21] 林晓光.物理法活性炭酸洗方法的改进[J]. 林产化工通讯, 2000(4):35-36.
[22] 丁雪梅, 徐向红, 邢沈阳, 等. SPSS 数据分析及 Excel 作图在毕业论文中的应用[J]. 实验室研究与探索, 2012(3): 122-128.Doi:10.3969/j.issn.1006-7167.2012.03.033. Ding X M, Xu X H, Xing S Y, et al. Application of data analysis by SPSS and figure construction by excel in the graduation thesis[J]. Research and Exploration in Laboratory, 2012(3): 122-128.
[23] 高尚愚,周建斌,宋林,等.碘值、亚甲基蓝及焦糖脱色力与活性炭孔隙结构的关系[J].南京林业大学学报, 1998, 22(4).Doi:10.3969/j.jssn.1000-2006.1988.04.005. Gao S Y, Zhou J B, Song L, et al. A study on the relationship between iodine number,methylene blue adsorption, caramel adsorption and the pore structure of activated carbons [J]. Journal of Nanjing Forestry University, 1998, 22(4).
[24] Dai J,Vendrame W A, Merkle S A, et al. Enhancing the productivity of hybrid yellow-poplar and hybrid sweet gum embryogenic cultures [J]. In vitro Cellular & Developmental Biology-Plant, 2004, 40(4): 376-383.Doi:10.1079/IVP2004538.
[25] George E F, Hall M A, De Klerk G-J. The components of plant tissue culture media II: organic additions, osmotic and pH effects, and support systems [C]// Plant Propagation by Tissue Culture.Netherlands: Springer, 2008:115-173.Doi:10.1007/978-1-1020-5005-3-4.
[26] Chin W Y W, Annuar M S M, Tan B C, et al. Evaluation of a laboratory scale conventional shake flask and a bioreactor on cell growth and regeneration of banana cell suspension cultures[J]. Scientia Horti Culturae, 2014, 172: 39-46.Doi:10.1016/j.scienta.2014.03.042.
[27] David L Smith, Abraham D Krikorian. Somatic proembryo production from excised, wounded zygoticcarrot embryos on hormone-free medium: evaluation of the effects of pH, ethylene and activated charcoal [J]. Plant Cell Reports, 1990, 9:34-37.Doi:10.10077/bf00232131.
[28] Thomas T D. The role of activated charcoal in plant tissue culture [J]. Biotechnology Advances, 2008, 26(6): 618-631.Doi:10.1016/j.biotechadv.2008.08.003.

不同活性炭对杂交鹅掌楸体胚发生的影响

Effects of different activated carbon on somatic embryogenesis of Liriodendron hybrids

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	杨永. 裸子植物的系统分类:历史、现状和展望[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 14-26.
[2]	张瑞, 周正虎, 王传宽, 金鹰. 东北温带森林不同材性树种木质部解剖和水力性状[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 229-236.
[3]	黄永健, 荀航, 张保, 尤俊昊, 姚曦, 汤锋. HPLC同时测定竹笋中8种酚酸类物质含量的方法研究及其应用[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 237-244.
[4]	邓云飞. 安息香科的系统学研究进展[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 27-35.
[5]	李家亮, 巫大宇, 毛康珊. 柏木属的分类地位和物种多样性研究现状与建议[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 36-45.
[6]	李涌福, 杨庆华, 陈林, 张敏, 向其柏, 王贤荣, 段一凡. 木犀属内分组关系的分类修订[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 58-62.
[7]	杨皓, 刘超, 庄家尧, 张树同, 张文韬, 毛国豪. 不同载体菌肥对紫穗槐生长和光合特性及土壤养分的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 81-89.
[8]	丁咏, 刘鑫, 张金池, 王宇浩, 陈美玲, 李涛, 刘孝武, 周悦湘, 孙连浩, 廖艺. 酸雨类型转变对杉木林地土壤和细根生长的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 90-98.
[9]	武燕, 黄青, 刘讯, 郑睿, 岑佳宝, 丁波, 张运林, 符裕红. 西南喀斯特地区马尾松人工林林龄对土壤理化性质的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 99-107.
[10]	卜晓婷, 付威, 李淑娴, 徐志标, 彭大庆, 徐林桥. 幼化和外源激素对娜塔栎嫩枝扦插生根的影响及其生根解剖学观察[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 129-136.
[11]	杜晋城, 李欣欣, 王泽亮, 刘偲, 钟毅, 王丽华. 聚乙二醇胁迫下3个油橄榄品种生理指标响应[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 137-143.
[12]	方静, 张书曼, 严善春, 武帅, 赵佳齐, 孟昭军. 两种丛枝菌根真菌复合接种对青山杨叶片抗美国白蛾的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 144-154.
[13]	张馨方, 王广鹏, 张树航, 李颖, 郭燕. 不同抗螨性板栗差异次生代谢物筛选与分析[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 234-240.
[14]	杨宏, 伊贤贵, 王贤荣, 吴桐, 周华近, 陈洁, 李蒙, 朱兆青. 樱花新品种‘元春’[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 275-276.
[15]	田梦阳, 朱树林, 窦全琴, 季艳红. 薄壳山核桃-茶间作对‘安吉白茶’速生期光合特性的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 86-96.