松材线虫体内细菌对宿主繁殖和致病力的影响

doi:10.3969/j.issn.1000-2006.2016.03.008

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (03): 47-51.doi: 10.3969/j.issn.1000-2006.2016.03.008

松材线虫体内细菌对宿主繁殖和致病力的影响

何龙喜^1,2,薛旗¹,吴小芹^1*

1. 南方现代林业协同创新中心, 南京林业大学林学院,江苏省有害生物入侵预防与控制重点实验室, 江苏南京 210037;
2. 江西省林业有害生物防治检疫局,江西南昌 330038

出版日期:2016-06-18 发布日期:2016-06-18
基金资助:
收稿日期:2015-10-17 修回日期:2016-03-20
基金项目:国家自然科学基金项目(31270683); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:何龙喜(longxihe@163.com)。*通信作者:吴小芹(xqwu@njfu.edu.cn),教授。
引文格式:何龙喜,薛旗,吴小芹. 松材线虫体内细菌对宿主繁殖和致病力的影响[J]. 南京林业大学学报(自然科学版),2016,40(3):47-51.

Effects of endobacteria on reproduction and virulence of Bursaphelenchus xylophilus

HE Longxi1,2, XUE Qi1, WU Xiaoqin1*

1.Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Pest Prevention and Management of Invasive specie, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. Jiangxi Forest Pest Control and Quarantine Bureau, Nanchang 330038, China

Online:2016-06-18 Published:2016-06-18

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摘要/Abstract

摘要： 为探讨松材线虫体内细菌在松材线虫病中的作用,对无菌松材线虫、体内细菌处理的松材线虫和野生型松材线虫的繁殖量和致病力进行了测定。结果表明:在非寄生条件下,无菌松材线虫的产卵量、卵孵化率和繁殖量显著高于携带细菌的松材线虫; 松材线虫体内寄生菌嗜麦芽窄食单孢菌NSPmBx03胞外代谢产物对松材线虫产卵量具有一定抑制作用。不同处理的松材线虫接种到3年生马尾松后,其繁殖量从高到低为野生型松材线虫>体内细菌处理的松材线虫>无菌松材线虫。对马尾松发病情况观察发现:接种野生型松材线虫的马尾松发病最早,病程最短; 次之为接种体内细菌处理的松材线虫; 最后是接种无菌松材线虫的处理。这表明嗜麦芽窄食单胞菌NSPmBx03对松材线虫在寄生和非寄生条件下繁殖的影响不同,对松材线虫致病力有一定增强作用。

Abstract: To investigate the role of endobacterium in the pine wilt disease, the reproduction and virulence of non-parasitic Bursaphelenchus xylophilus, B. xylophilus treated with endobactria and wild-type B. Xylophilus were measured. We found that the spawning number, hatching rate and reproduction number of aseptic B. xylophilus was higher than B. xylophilus treated with bacteria in non-parasitic condition. Extracellular metabolism of endobacterium could inhibit the reproduction of B. xylophilus. After inoculated with 3 years old Pinus massoniana, the reproduction number of nematodes from high to low was as follow:wild-type B. xylophilus>B. xylophilus treated with endobacterium> aseptic B. xylophilus. The pines that inoculated with wild-type B. xylophilus firstly occurred wilting and the course of disease was the shortest in the three different treatments of B. xylophilus. Secondly, the B. xylophilus treated with endobacteria appeared wilting, and the aseptic B. xylophilus appeared lastly. These results indicated that endobacterium exerted different effect on the development of B. xylophilus in non-parasitic and parasitism condition and could enhance the virulence of nematode.

中图分类号:

S763.16

引用本文

何龙喜,薛旗,吴小芹. 松材线虫体内细菌对宿主繁殖和致病力的影响[J]. 南京林业大学学报（自然科学版）, 2016, 40(03): 47-51.

HE Longxi1,2, XUE Qi1, WU Xiaoqin1*. Effects of endobacteria on reproduction and virulence of Bursaphelenchus xylophilus[J].Journal of Nanjing Forestry University (Natural Science Edition), 2016, 40(03): 47-51.DOI: 10.3969/j.issn.1000-2006.2016.03.008.

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[1]	潘妍毅,黄麟,吴小芹. 拟松材线虫组织蛋白酶基因Bmcath1的序列分析与转录水平检测[J]. 南京林业大学学报（自然科学版）, 2015, 39(06): 12-16.
[2]	袁为敏,项杨,吴小芹. 拟松材线虫不同致病力虫株体内细菌的种类差异[J]. 南京林业大学学报（自然科学版）, 2015, 39(04): 63-68.
[3]	许峻荣,吴小芹,刘云,叶建仁. 基于松材线虫全基因组序列的SSR标记开发[J]. 南京林业大学学报（自然科学版）, 2014, 38(02): 36-42.
[4]	史延梅，陈凤毛，叶建仁，方国飞，潘宏阳. 我国松树寄生线虫分类检索[J]. 南京林业大学学报（自然科学版）, 2012, 36(01): 137-141.