Abstract: 【Objective】Evaluate the growth characteristics and fruit quality of ‘Zhaixuan’ blueberry strains No. 3, No. 4, No. 5, No. 7, No. 8, No. 9 at Nanjing in order to screen superior breeding materials. 【Method】 The growth and fruiting characteristics including growth amount, yield, flowering and fruiting phenophase, and fruit quality of six ‘Zhaixuan’ blueberry strains were observed, and evaluated in according to blueberry breeding goals. 【Result】All the six ‘Zhaixuan’ blueberry strains were early mature, high-yielding, with excellent adaptability in Nanjing area of China. The fruit yields of the five-year-old No. 4, No. 7, No. 3 and No. 9 strains exceeded 3.40 kg per plant, the fruit matured in late May or early June, fruit hardness was around 2.00 kg/cm², and the polyphenol and anthocyanin content of the fruits was very high. The No. 7 strain produced the earliest maturing fruit, which had a good flavor, the highest anthocyanin and polyphenol content and fresh fruit sensory score. The No. 4 strain had relatively high growth, anthocyanin and polyphenol content, the highest fruit hardness and yield per plant, and the second-highest fresh fruit sensory score. 【Conclusion】The strains ‘Zhaixuan’ No. 7 and No. 4 were comprehensively evaluated as potential candidates for excellent blueberry cultivar.