基于SSR标记构建宝巾花品种的分子指纹

doi:10.3969/j.issn.1000-2006.201812045

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南京林业大学学报（自然科学版） ›› 2019, Vol. 43 ›› Issue (6): 60-66.doi: 10.3969/j.issn.1000-2006.201812045

基于SSR标记构建宝巾花品种的分子指纹

孙利娜¹^,²(), 周群³, 黄妹平², 周长品¹, 徐世松⁴, 杨舒婷², 杜铃², 甘四明¹^,²^,^*(), 唐庆²^,^*()

1.中国林业科学研究院热带林业研究所, 热带林业研究国家林业局重点实验室, 广东广州 510520
2.广西壮族自治区林业科学研究院, 广西优良用材林资源培育重点实验室, 中南速生材繁育国家林业局重点实验室, 广西南宁 530002
3.厦门市园林植物园, 福建厦门 361003
4.中国热带农业科学院热带作物品种资源研究所, 海南儋州 571737

收稿日期:2018-12-26 修回日期:2019-06-24 出版日期:2019-11-30 发布日期:2019-11-30
通讯作者: 甘四明,唐庆
基金资助:
广西科技计划项目(桂科AD17129021);“广西主要用材林资源高效培育与利用人才小高地”专项(桂财社函[2018]112号)

Using the SSR fluorescent labling to establish SSR fingerprint for 131 cultivars in Bougainvillea (Nyctaginaceae)

SUN Lina¹^,²(), ZHOU Qun³, HUANG Meiping², ZHOU Changpin¹, XU Shisong⁴, YANG Shuting², DU Ling², GAN Siming¹^,²^,^*(), TANG Qing²^,^*()

1. Key Laboratory of State Forestry Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China
2. Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation & Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Guangxi Forestry Research Institute, Nanning 530002, China
3. Xiamen Botanical Garden, Xiamen 361003, China
4. Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Received:2018-12-26 Revised:2019-06-24 Online:2019-11-30 Published:2019-11-30
Contact: GAN Siming,TANG Qing

摘要/Abstract

摘要：

【目的】针对宝巾花(Bougainvillea)品种繁多、同物异名和同名异物多有发生的现象,利用可靠的分子标记技术进行品种的有效分类。【方法】基于15个简单重复序列(SSR)位点,利用荧光标记的引物对131个宝巾花品种进行PCR扩增,扩增产物经毛细管电泳检测,分析品种多样性和遗传距离,并构建品种聚类图和指纹图谱。【结果】15个SSR位点共扩增出85个等位基因,平均每个位点的等位片段数5.60个,Shannon’s 信息指数、观测杂合度和期望杂合度的变化范围分别为0.38~1.53、0.11~0.94和0.16~0.73,平均值分别为1.04、0.50和0.57,位点的多态性信息量介于0.15~0.69之间,平均0.51。宝巾花品种的遗传距离为0.00~0.65,平均0.33;聚类分析表明,同一种的品种大多数聚在一类,但同一个种仍有品种未聚在一类或亚类,也有多个种的品种聚在一类;有多对品种存在同物异名或同名异物的现象;基于11个位点可有效鉴别131个品种,建立了各品种的分子指纹图谱。【结论】对同物异名或同名异物的品种,需要进一步确认品种中文名或拉丁名;基于SSR标记的宝巾花品种的指纹图谱为宝巾花品种登记、注册和知识产权保护提供了可靠技术,也为品种鉴定提供了有效手段。

关键词: 宝巾花, 品种分类, SSR标记, 指纹图谱, 亲缘关系

Abstract:

【Objective】 Find a reliable molecular marker to identifyBougainvillea cultivars that are difficult to distinguish, due to cultivar richness and cultivar homonymy and synonymy. 【Method】 A total of 15 simple sequence repeats (SSR) markers were used to amplify DNA samples from 131 Bougainvillea cultivars. SSR amplicons were detected by a capillary electrophoresis method. Cultivar polymorphism and genetic distance were calculated; they were used for clustering analysis and fingerprint construction of all the 131 cultivars.【Result】A total of 85 different alleles were collectively generated by the 15 SSR loci, averaging 5.60 alleles per locus. Shannon’s information index, observed heterozygosity value, and expected heterozygosity value ranged from 0.38 to 1.53, 0.11 to 0.94 and 0.16 to 0.73, with an average of 1.04, 0.50 and 0.57 per locus, respectively. The polymorphism information content ranged from 0.15 to 0.69, with an average of 0.51 per locus. The genetic distance between cultivars ranged from 0.00 to 0.65, with an average of 0.33. Clustering analysis showed that most of the cultivars within a species tended to fall in the same cluster, but some cultivars of the same, whereas certain cultivars from different species were grouped in the same cluster. Several pairs of cultivars were either homonyms or synonyms. Only 11 SSR loci were shown to be effective for identifying the 131 cultivars; thus, the molecular fingerprints of all these cultivars were constructed based on allelic combinations of the 11 markers. 【Conclusion】Further research should be conducted to verify either the Chinese or Latin names of some suspicious cultivars. SSR-based fingerprints ofBougainvillea cultivars constitute a reliable technique for cultivar identification, registration, and intellectual property protection as well as for cultivar clarification in production practices.

Key words: Bougainvillea, cultivar classitication, SSR, fingerprints, genetic relationship

中图分类号:

孙利娜,周群,黄妹平,等. 基于SSR标记构建宝巾花品种的分子指纹[J]. 南京林业大学学报（自然科学版）, 2019, 43(6): 60-66.

SUN Lina, ZHOU Qun, HUANG Meiping, ZHOU Changpin, XU Shisong, YANG Shuting, DU Ling, GAN Siming, TANG Qing. Using the SSR fluorescent labling to establish SSR fingerprint for 131 cultivars in Bougainvillea (Nyctaginaceae)[J].Journal of Nanjing Forestry University (Natural Science Edition), 2019, 43(6): 60-66.DOI: 10.3969/j.issn.1000-2006.201812045.

图/表 3

表1

参试的131个宝巾花品种"

序号 No.	品种 cultivar	序号 No.	品种 cultivar	序号 No.	品种 cultivar
1	‘青叶紫’(‘小叶浅紫’) (B. glabra ‘Elizabeth Angus’)	45	‘斑叶小叶紫’(B. × buttiana ‘Sanderiana Variegata’)	89	‘粉蝶’(B. sp. ‘Feathery Fantasy Double Colour’)
2	‘粉粧’(B. glabra ‘Eva’)	46	‘宝花橙’(B. × buttiana ‘Louise Wathen’)	90	‘红蝶’(B. sp. ‘Ratana Pink’)
3	‘黄金叶’(B. glabra ‘Golden Lady’)	47	‘彩虹黄’(B. × buttiana ‘Yellow Wonder’)	91	‘红莲花’(B. sp. ‘Red Lotus’)
4	‘茄色’(B. glabra ‘Merial Fitzpartick’)	48	‘橙冰’(B. × buttiana ‘Louise Wathen Variegata’)	92	‘黄金大奖’(B. sp. ‘MonSam’)
5	‘塔紫’(B. glabra ‘Pink Pixie’)	49	‘红粉佳人’(B. × buttiana ‘Alick Lancaste’)	93	‘绿叶玉宝’(B. sp. ‘Ladybird’)
6	‘水红’(B. glabra ‘Rosa’)	50	‘加州黄金’(B. × buttiana ‘California Gold’)	94	‘蒙娜丽莎黄’(B. sp. ‘Mona Lisa Yellow’)
7	‘光叶斑叶紫花’(B. glabra ‘Variegata’)	51	‘金边叶重粉’(B. × buttiana ‘Los Banos Variegata’)	95	‘孟加拉红’(B. sp. ‘Royal Bengal Red’)
8	‘橙红’(B. spectabilis ‘Auratus’)	52	‘金发女郎’(B. × buttiana ‘Blondie’)	96	‘热带花’(B. sp. ‘Tropical Bouquet’)
9	‘胭脂红’(B. spectabilis ‘China Beauty’)	53	‘蓝月亮’(B. × buttiana ‘Blue Moon’)	97	‘塔黄’(B. sp. ‘Pixie Yellow’)
10	‘大花深红’(B. spectabilis ‘Crimonlake’)	54	‘绿叶橙’(B. × buttiana ‘Afterglow’)	98	‘蜥蜴橙’(B. sp. ‘Lguana Variegata’)
11	‘大花深紫’(B. spectabilis ‘Senjakala’)	55	‘绿叶柠檬黄’(B. × buttiana ‘Golden Glow’)	99	‘小粉雀’(B. sp. ‘Firecracker Purple’)
12	‘樱花’(B. × spectoglabra ‘Imperial Delight’)	56	‘水红’(B. × buttiana ‘Miss Manila’)	100	‘小金雀’(B. sp. ‘Firecracker Yellow’)
13	‘金心’(B. × spectoglabra ‘Thimma’)	57	‘香港丽人’(B. × buttiana ‘Hong Kong Beauty’)	101	‘银边叶浅紫’(B. sp. ‘Pixie Orange’)
14	‘皱叶深红’(B. × buttiana ‘Barbara Karst’)	58	‘小花大红’(B. × buttiana ‘Mrs. Butt’)	102	‘暗斑叶加州黄金’(B. sp.)
15	‘暗斑叶橙红’(B. × buttiana ‘Lateritia’)	59	‘猩红皇后’(B. × buttiana ‘San Diego Red’)	103	‘冰斑美人’(B. sp.)
16	‘柠檬黄’(B. × buttiana ‘Mrs McLean’)	60	‘怡锦’(B. × buttiana ‘Cherry Blossom’)	104	‘波依斯玫瑰’(B. sp.)
17	‘重瓣大红’(B. × buttiana ‘Pretoria’)	61	‘印度画报’(B. × buttiana ‘Chitra’)	105	‘大蜜叶精灵’(B. sp.)
18	‘斑叶塔紫’(B. × buttiana ‘Temple Fire’)	62	‘重斑粉’(B. × buttiana ‘Los Banos Beauty’)	106	‘红宝石’(B. sp.)
19	‘福摩斯’(B. glabra ‘Formosa’)	63	‘重瓣橙’(B. × buttiana ‘Roseville’s Delight’)	107	‘红粉夫人’(B. sp.)
20	‘安格斯’(B. glabra ‘Elizabeth Angus’)	64	‘重瓣红’(B. × buttiana ‘Mahara’)	108	‘红九月’(B. sp.)
21	‘暗斑叶白’(B. glabra ‘Bambino Beesnees’)	65	‘重瓣黄’(B. × buttiana ‘Aussie Gold’)	109	‘红叶大红’(B. sp.)
22	‘白雪公主’(B. glabra ‘Alba’)	66	‘重瓣枣红’(B. × buttiana )	110	‘金边小叶紫’(B. sp.)
23	‘斑叶怡锦’(B. glabra ‘Mrs. Eusenia Raja Singhe’)	67	‘重瓣白’(B. × buttiana )	111	‘金龟紫’(B. sp.)
24	‘光叶紫’(B. glabra ‘Sanderiana’)	68	‘帕塔’(B. peruviana ‘Partha’)	112	‘金叶浅粉’(B. sp.)
25	‘金边安格斯’(B. glabra ‘Elizabeth Angus Variegata’)	69	‘斑叶印度橙粉’(B. peruviana ‘Partha Variegata’)	113	‘拉菲泰’(B. sp.)
26	‘金边叶浅紫’(B. glabra ‘Mrs. Eva Variegata Mauve II’)	70	‘红心樱花’(B. peruviana ‘Makris’)	114	‘绿叶浅黄’(B. sp.)
27	‘金叶白’(B. glabra ‘JinYu’)	71	‘金双色’(B. peruviana ‘Thimma’)	115	‘浅色樱花’(B. sp.)
28	‘哭泣的美人’(B. glabra ‘Variegata’)	72	‘口红’(B. peruviana ‘Begum Sikander’)	116	‘仨斑叶紫’(B. sp.)
29	‘柳叶白’(B. glabra ‘Shweta’)	73	‘绿叶宫粉’(B. peruviana ‘Mrs. H.C. Buck’)	117	‘洒金叶橙’(B. sp.)
30	‘绿叶浅紫’(B. glabra ‘Mrs. Eva’)	74	‘蒙娜丽莎’(B. peruviana ‘Mona Lisa’)	118	‘沙瓣叶艳红’(B. sp.)
31	‘塔红’(B. glabra ‘Pink Pixie’)	75	‘洋红公主’(B. peruviana ‘Mrs. H.C. Buck’)	119	‘沙瓣叶紫’(B. sp.)
32	‘小叶紫’(B. glabra ‘Royal Purple’)	76	‘印度橙粉’(B. peruviana ‘Partha’)	120	‘莎莎’(B. sp.)
33	‘新加坡白’(B. glabra ‘Singapore White’)	77	‘樱花’(B. peruviana ‘Imperial Delight’)	121	‘索菲亚’(B. sp.)
34	‘新加坡粉’(B. glabra ‘Singapore Beauty’)	78	‘火焰’(B. spectabilis ‘Flame’)	122	‘顽皮’(B. sp.)
35	‘雪紫’(B. glabra ‘Snow Purple’)	79	‘毛叶’(B. spectabilis ‘Splendens’)	123	‘小花粉紫’(绿叶) (B. sp.)
36	‘牙买加白’(B. glabra ‘Jamaica White’)	80	‘玫红’(B. spectabilis ‘B.J.F. Bailey’)	124	‘小叶小红粉’(B. sp.)
37	‘胭脂红’(B. glabra ‘Zinia Barat’)	81	‘热火桑巴’(B. spectabilis ‘Flame’)	125	‘异叶红’(B. sp.)
38	‘银边叶白’(B. glabra ‘Mrs. Eva Variegata White’)	82	‘小红雀’(B. spectabilis ‘Tomato Red’)	126	‘银河’(B. sp.)
39	‘银河’(B. glabra ‘Galaxy’)	83	‘婴儿玫瑰’(B. spectabilis ‘Baby Rose’)	127	‘银沙丽人’(B. sp.)
40	‘紫蝶’(B. glabra ‘Mrs. Eva Purple Ratana’)	84	‘砖红’(B. spectabilis ‘Lateritia’)	128	‘杂斑叶大红’(B. sp.)
41	‘紫丁香美女’(B. glabra ‘Lilac Beauty’)	85	‘暗斑叶樱花’(B. sp. ‘Blushing Beauty’)	129	‘杂斑叶水红’(B. sp.)
42	‘暗斑叶橙’(B. × buttiana ‘Jinda Orange’)	86	‘斑叶塔橙’(B. sp. ‘Pixie Orange Variegata’)	130	‘紫叶布丁’(B. sp.)
43	‘暗斑叶红’(B. × buttiana ‘Juanita Hatten’)	87	‘橙灯笼’(B. sp. ‘Chinese Lantern’)	131	‘总统紫’(B. sp.)
44	‘巴特夫人’(B. × buttiana ‘Mrs. Butt’)	88	‘橙蝶’(B. sp. ‘Ratana Orange’)

表1

表2

图1

参考文献 22

[1]	周群. 三角梅的品种分类[M]. 北京: 金盾出版社, 2009.
	ZHOU Q. Bougainvillea cultivation and appreciation [M]. Beijing: Golden Shield Press, 2009.
[2]	周群. 中国叶子花属植物种质资源及其繁殖技术研究[J]. 中国农学通报, 2008, 24(12):321-324.
	ZHOU Q. Research on germplasm resource and propagational technique of Bougainvillea L. in China [J]. Chinese Agricultural Science Bulletin, 2008, 24(12):321-324.
[3]	周群, 黄克福, 丁印龙, 等. 中国引栽三角梅属观赏品种的调查与分类鉴定[J]. 江西农业学报, 2011, 23(5):53-56. DOI: 10.19386/j.cnki.jxnyxb.2011.05.017.
	ZHOU Q, HUANG K F, DING Y L, et al. Investigation and taxonomic identification on introduced ornamental varieties in Bougainvillea in China [J]. Acta Agriculturae Jiangxi, 2011, 23(5):53-56.
[4]	陈瑞阳, 宋文芹, 李秀兰, 等. 中国主要经济植物基因组染色体图谱(第三册): 中国园林花卉植物染色体图谱[M]. 北京: 科学出版社, 2003.
	CHEN R Y, SONG W Q, LI X L, et al. Chromosome atlas of major economic plants genome in China, III [M]. Beijing: Science Press, 2003.
[5]	孙利娜, 王华新, 龚建英, 等. 宝巾花辐射诱变效应研究初报[J]. 北方园艺, 2013(19):91-93.
	SUN L N, WANG H X, GONG J Y, et al. Preliminary study on the mutagenic effects of radiation on Bougainvillea glabra [J]. Northern Horticulture, 2013(19):91-93.
[6]	JARNE P, LAGODA P J L. Microsatellites, from molecules to populations and back[J]. Trends in Ecology and Evolution, 1996, 11(10):424-429. DOI: 10.1016/0169-5347(96)10049-5. doi: 10.1016/0169-5347(96)10049-5
[7]	KALIA R K, RAI M K, KALIA S, et al. Microsatellite markers: an overview of the recent progress in plants[J]. Euphytica, 2011, 177(3):309-334. DOI: 10.1007/s10681-010-0286-9. doi: 10.1007/s10681-010-0286-9
[8]	程本义, 夏俊辉, 龚俊义, 等. SSR 荧光标记毛细管电泳检测法在水稻 DNA 指纹鉴定中的应用[J]. 中国水稻科学, 2011, 25(6):672-676. DOI: 10.3969/j.issn.1001-7216.2011.06.016.
	CHENG B Y, XIA J H, GONG J Y, et al. Application of capillary electrophoresis detection with fluorescent SSR markers in rice DNA fingerprint identification[J]. Chinese Journal of Rice Science, 2011, 25(6):672-676.
[9]	高源, 刘凤之, 王昆, 等. 基于TP-M13-SSR 指纹图谱的中国原产苹果属植物分子身份证的建立[J]. 植物遗传资源学报, 2015, 16(6):1290-1297. DOI: 10.13430/j.cnki.jpgr.2015.06.022.
	GAO Y, LIU F Z, WANG K, et al. Establishment of molecular identity card for Malus Mill. originated from China based on the fingerprints of TP-M13-SSR [J]. Journal of Plant Genetic Resources, 2015, 16(6):1290-1297.
[10]	毛秀红, 郑勇奇, 孙百友, 等. 基于SSR的刺槐无性系遗传多样性分析和指纹图谱构建[J]. 林业科学, 2017, 53(10):80-89. DOI: 10.11707/j.1001-7488.20171009.
	MAO X H, ZHENG Y Q, SUN B Y, et al. Genetic diversity and fingerprints of Robinia pseudoacacia clones based on SSR markers [J]. Scientia Silvae Sinicae, 2017, 53(10):80-89.
[11]	段一凡, 王贤荣, 梁丽丽, 等. 桂花品种SSR荧光指纹图谱的构建[J]. 南京林业大学学报(自然科学版), 2014, 38(S1):1-6. DOI: 10.3969/j.issn.1000-2006.2014.S1.001.
	DUAN Y F, WANG X R, LIANG L L, et al. Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers [J]. ournal of Nanjing Forestry University (Natural Sciences Edition), 2014, 38(S1):1-6.
[12]	王龙, 薛华柏, 杨健, 等. 80个梨品种SSR特征指纹数据表的构建[J]. 果树学报, 2016, 33(增刊):43-51. DOI: 10.13925/j.cnki.gsxb.2016.S.05.
	WANG L, XUE H B, YANG J, et al. Construction of SSR characteristic fingerprinting data table for 80 pear cultivars[J]. Journal of Fruit Science, 2016, 33(supplement):43-51.
[13]	王童欣, DAVID E A, 罗灿, 等. 基于 SSR 分子标记技术的欧榛品种遗传多样性分析[J]. 2018, 16(19):6415-6420. DOI: 10.13271/j.mpb.016.006415.
	WANG T X, DAVID E A, LUO C, et al. Genetic diversity analysis of Corylus avellana L. cultivars based on SSR molecular marker technology [J]. Molecular Plant Breeding, 2018, 16(19):6415-6420.
[14]	RICHA S, SUDHIR S, ARVIND S, et al. RAPD-based genetic relationships in different Bougainvillea cultivars[J]. Crop Breeding and Applied Biotechnology, 2009(9):154-163. DOI: 10.12702/1984-7033.v09n02a08.
[15]	李房英, 黄彦晶, 吴少华. 三角梅种质资源的ISSR分析[J]. 热带作物学报, 2011, 32(9):1692-1696. DOI: 10.3969/j.issn.1000-2561.2011.09.022.
	LI F Y, HUANG Y J, WU S H. ISSR analysis of germplasm resources of Bougainvillea spectabilis Willd [J]. Chinese Journal of Tropical Crops, 2011, 32(9):1692-1696.
[16]	孙利娜, 林茂, 陈升侃, 等. 宝巾花基于转录组测序的SSR标记的开发和应用[J/OL]. 分子植物育种, 1-16 [2019- 11- 13]. http://kns.cnki.net/kcms/detail/46.1068.s:20191011.1624.006.html.
	SUN L N, LIN M, CHEN S K, et al. Development and application of SSR markers derived from RNAseq data in Bougainvillea (Nyctaginaceae)[J]. Molecular Plant Breeding, 1-16 [2019- 11- 13]. http://kns.cnki.net/kcms/detail/46.1068.s:20191011.1624.006.html.
[17]	LIU K, MUSE S V. PowerMarker: an integrated analysis environment for genetic marker analysis[J]. Bioinformatics, 2005, 21(9):2128-2129. DOI: 10.1093/bioinformatics/bti282. doi: 10.1093/bioinformatics/bti282
[18]	PEAKALL R, SMOUSE P. GenAlEx 6: genetic analysis in excel: population genetic software for teaching and research[J]. Molecular Ecology Notes, 2006, 6(1):288-295. DOI: 10.1111/j.1471-8286.2005.01155.x. doi: 10.1111/men.2006.6.issue-1
[19]	ROHLF F J. NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1 [EB/OL]. [2017- 01- 12]. http://www.exetersoftware.com/cat/ntsyspc/ntsyspc.html.
[20]	郝晨阳, 王兰芬, 贾继增, 等. SSR 荧光标记和银染技术的比较分析[J]. 作物学报, 2005, 31(2):144-149. DOI: 10.3321/j.issn:0496-3490.2005.02.002.
	HAO C Y, WANG L F, JIA J Z, et al. Comparison of fluorescence and silver-staining detection systems of microsatellite markers[J]. Acta Agronomica Sinica, 2005, 31(2):144-149.
[21]	李莉, 杨剑波, MACKILL D J, 等. 水稻 SSR 不同检测和分析方法的比较研究[J]. 中国水稻科学, 2000, 14(3):185-188. DOI: 10.16819/j.1001-7216.2000.03.015.
	LI L, YANG J B, MACKILL D J, et al. Comparison of different detection methods for rice SSR analysis[J]. China J Rice Sci, 2000, 14(3):185-188.
[22]	刘新龙, 马丽, 陈学宽, 等. 云南甘蔗自育品种 DNA 指纹身份证构建[J]. 作物学报, 2010, 36(2):202-210. DOI: 10.3724/SP.J.1006.2010.00202.
	LIU X L, MA L, CHEN X K, et al. Establishment of DNA fingerprint ID in sugarcane cultivars in Yunnan, China[J]. Acta Agronomica Sinica, 2010, 36(2):202-210.

EST-SSR引物 EST-SSR primer	N_a	N_e	I	H_o	H_e	PIC
BOUeSSR01	8.00	3.36	1.42	0.91	0.70	0.65
BOUeSSR02	3.00	1.98	0.76	0.15	0.49	0.39
BOUeSSR03	5.00	2.37	1.01	0.37	0.58	0.49
BOUeSSR04	9.00	3.63	1.53	0.49	0.73	0.69
BOUeSSR05	7.00	3.34	1.39	0.44	0.70	0.66
BOUeSSR06	6.00	2.50	1.07	0.84	0.60	0.53
BOUeSSR08	4.00	3.08	1.16	0.94	0.68	0.61
BOUeSSR09	4.00	2.33	0.96	0.78	0.57	0.48
BOUeSSR10	4.00	1.83	0.78	0.16	0.45	0.39
BOUeSSR11	7.00	2.28	1.12	0.58	0.56	0.51
BOUeSSR12	4.00	1.90	0.70	0.14	0.47	0.37
BOUeSSR13	5.00	1.19	0.38	0.11	0.16	0.15
BOUeSSR14	4.00	2.91	1.10	0.84	0.66	0.58
BOUeSSR15	6.00	2.59	1.15	0.60	0.61	0.56
BOUeSSR16	9.00	2.58	1.15	0.20	0.61	0.54
均值mean	5.60	2.52	1.04	0.50	0.57	0.51

基于SSR标记构建宝巾花品种的分子指纹

Using the SSR fluorescent labling to establish SSR fingerprint for 131 cultivars in Bougainvillea (Nyctaginaceae)

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