雄全异株桂花不同花期光合和内源激素的变化

doi:10.12302/j.issn.1000-2006.202107032

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南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (5): 75-80.doi: 10.12302/j.issn.1000-2006.202107032

雄全异株桂花不同花期光合和内源激素的变化

张成¹^,²(), 王小燕¹^,²(), 王贤荣¹^,²^,^*(), 段一凡¹^,²^,^*(), 张敏¹^,², 施大伟¹, 朱跃¹^,², 宋炎峰¹^,², 柴子涵¹^,², 李岚¹^,²

1.南京林业大学生物与环境学院,南方现代林业协同创新中心,江苏南京 210037
2.木犀属植物栽培品种国际登录中心,江苏南京 210037

收稿日期:2021-07-20 修回日期:2021-11-28 出版日期:2022-09-30 发布日期:2022-10-19
通讯作者: 王贤荣,段一凡
基金资助:
江苏省研究生科研与实践创新计划项目(KYCX19_1073);江苏省自然科学基金项目(BK20200786);南京林业大学青年学者创新基金项目(CX2019029);中国博士后科学基金项目(2019M651839)

Photosynthesis and hormone study of male and hermaphroditic Osmanthus fragrans at different flowering stages

ZHANG Cheng¹^,²(), WANG Xiaoyan¹^,²(), WANG Xianrong¹^,²^,^*(), DUAN Yifan¹^,²^,^*(), ZHANG Min¹^,², SHI Dawei¹, ZHU Yue¹^,², SONG Yanfeng¹^,², CHAI Zihan¹^,², LI Lan¹^,²

1. College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
2. International Cultivar Registration Center for Osmanthus, Nanjing 210037, China

Received:2021-07-20 Revised:2021-11-28 Online:2022-09-30 Published:2022-10-19
Contact: WANG Xianrong,DUAN Yifan

摘要/Abstract

摘要：

【目的】桂花(Osmanthus fragrans)同时拥有雄株和两性株,具有极为罕见的雄全异株繁育系统。对桂花不同花期的光合特性及花部组织的内源激素含量进行测定,比较雄性和两性桂花在不同花期的光合及内源激素含量的变化规律,为评价雄性和两性桂花生长适应性及后续生理生态学研究提供参考。【方法】利用Li-6400XT光合作用测量系统对雄性和两性桂花不同花期的光合特性进行测量,并利用酶联免疫吸附分析法对生长素(IAA)、茉莉酸(JA)、脱落酸(ABA)、赤霉素(GA₃)和玉米素核苷(ZRs)5种植物激素含量进行测定。【结果】雄性桂花的气孔导度和净光合速率在不同花期均低于两性桂花的;测定的5种激素中,平均激素含量最高的为脱落酸;茉莉酸含量在雄花的花期内持续增加,玉米素核苷含量在两性花的花期内不断升高。【结论】气孔导度是影响桂花净光合速率的一个关键因子,与净光合速率呈正相关;脱落酸和茉莉酸的不断积累可能导致了雄性桂花花瓣的大量脱落;玉米素核苷在两性桂花花期的积累对果实的发育有促进作用。研究结果可为雄全异株桂花不同花期阶段提供基础生理数据,为后续探索雄花花瓣脱落及果实发育提供参考。

关键词: 桂花, 光合特性, 植物激素, 雄全异株, 花期

Abstract:

【Objective】Osmanthus fragrans with both male and hermaphroditic plants, possesses an extremely rare breeding system of androdioecy. The photosynthetic characteristics and endogenous hormone content of O. fragrans at different flowering stages were measured, and the changes of photosynthesis and endogenous hormone content in male and hermaphroditic O. fragrans at different flowering stages were compared, providing references for the evaluation of growth adaptability and subsequent physiological and ecological research of male and hermaphroditic O. fragrans.【Method】The photosynthetic characteristics of male and hermaphroditic flowers at different flowering stages were measured by Li-6400XT photosynthetic measurement system, and five plant hormones including auxin (IAA), jasmonic acid (JA), abscisic acid (ABA), gibberellin (GA₃) and zeatin nucleosides (ZRs) were measured by the enzyme-linked immunosorbent assay.【Result】The stomatal conductance and net photosynthetic rate of males were lower than that of hermaphroditic at different flowering stages. ABA had the highest hormone content among the five measured hormones. The content of JA continued to increase during the flowering period of male flowers and the content of ZRs continued to increase during the flowering period of hermaphroditic flowers.【Conclusion】Stomatal conductance was a key environmental factor affecting the net photosynthetic rate of O. fragrans, and was positively correlated with the net photosynthetic rate. The continuous accumulation of abscisic acid and jasmonic acid may lead to the mass abscission of male osmanthus petals. The accumulation of ZRs in the flowering stage of hermaphroditic flowers promoted the fruit development. The results provided basic physiological data for the different flowering stages of androdioecious O. fragrans, and provided references for the subsequent exploration of the mechanism of male flower petal abscission and fruit development.

Key words: Osmanthus fragrans, photosynthesis characteristics, plant hormone, androdioecy, flowering stage

中图分类号:

S718

张成,王小燕,王贤荣,等. 雄全异株桂花不同花期光合和内源激素的变化[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 75-80.

ZHANG Cheng, WANG Xiaoyan, WANG Xianrong, DUAN Yifan, ZHANG Min, SHI Dawei, ZHU Yue, SONG Yanfeng, CHAI Zihan, LI Lan. Photosynthesis and hormone study of male and hermaphroditic Osmanthus fragrans at different flowering stages[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(5): 75-80.DOI: 10.12302/j.issn.1000-2006.202107032.

图/表 4

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参考文献 25

[1]	郝日明, 赵宏波, 王金虎, 等. 野生桂花繁育系统的观察和研究[J]. 植物资源与环境学报, 2011, 20(1):17-24.
	HAO R M, ZHAO H B, WANG J H, et al. Observation and study on breeding system of wild Osmanthus fragrans[J]. J Plant Resour Environ, 2011, 20(1):17-24.DOI:10.3969/j.issn.1674-7895.2011.01.003.
[2]	XU Y C, ZHOU L H, HU S Q, et al. The differentiation and development of pistils of hermaphrodites and pistillodes of males in androdioecious Osmanthus fragrans L.and implications for the evolution to androdioecy[J]. Plant Syst Evol, 2014, 300(5):843-849.DOI:10.1007/s00606-013-0923-6.
[3]	汤文华, 窦全琴, 潘平平, 等. 不同薄壳山核桃品种光合特性研究[J]. 南京林业大学学报(自然科学版), 2020, 44(3):81-88.
	TANG W H, DOU Q Q, PAN P P, et al. Photosynthetic characteristics of grafted plants of different Carya illinoinensis varieties[J]. J Nanjing For Univ (Nat Sci Ed), 2020, 44(3):81-88.DOI: 10.3969/j.issn.1000-2006.201903004.
[4]	圣倩倩, 戴安琪, 宋敏, 等. NO₂胁迫下两种鹅耳枥的光合生理特性变化[J]. 南京林业大学学报(自然科学版), 2021, 45(2):10-16.
	SHENG Q Q, DAI A Q, SONG M, et al. Photosynthetic physiological characteristics of two kinds of hornbeam under NO₂ stress[J]. J Nanjing For Univ (Nat Sci Ed), 2021, 45(2):10-16.DOI:10.12302/j.issn.1000-2006.202006062.
[5]	SONG S S, QI T C, HUANG H, et al. Regulation of stamen development by coordinated actions of jasmonate,auxin,and gibberellin in Arabidopsis[J]. Mol Plant, 2013, 6(4):1065-1073.DOI:10.1093/mp/sst054.
[6]	YUAN Z, ZHANG D. Roles of jasmonate signalling in plant inflorescence and flower development[J]. Curr Opin Plant Biol, 2015, 27:44-51.DOI:10.1016/j.pbi.2015.05.024.
[7]	顾梨. 钝叶柃(Eurya obtusifolia)花器官发育及外源6-BA对其性别分化的影响研究[D]. 重庆: 西南大学, 2019.
	GU L. Study on floral organogenesis and effect of exogenous 6-BA on sex differentiation of Eurya obtusifolia[D]. Chongqing: Southwest University, 2019.
[8]	PAK H, GUO Y, CHEN M, et al. The effect of exogenous methyl jasmonate on the flowering time,floral organ morphology,and transcript levels of a group of genes implicated in the development of oilseed rape flowers (Brassica napus L.)[J]. Planta, 2009, 231(1):79-91.DOI:10.1007/s00425-009-1029-9.
[9]	汤正辉, 沈植国, 罗颖, 等. 遮荫对凤丹白光合特性的影响[J]. 西北林学院学报, 2017, 32(5):46-51.
	TANG Z H, SHEN Z G, LUO Y, et al. Effects of shading on the photosynthetic characteristics of Paeonia ostii cv.‘Phoenix white’[J]. J Northwest For Univ, 2017, 32(5):46-51.DOI: 10.3969/j.issn.1001-7461.2017.05.09.
[10]	赵靓, 杨佳鑫, 禹世豪, 等. 盐胁迫下嫁接对梅花光合生理特性和叶绿素荧光参数的影响[J]. 西北林学院学报, 2019, 34(6):43-48.
	ZHAO L, YANG J X, YU S H, et al. Effects of grafting on the photosynthetic physiological characteristic and chlorophyll fluorescence parameters of Prunus mume under salt stress[J]. J Northwest For Univ, 2019, 34(6):43-48.DOI: 10.3969/j.issn.1001-7461.2019.06.07.
[11]	ZHAO J, LI G, YI G X, et al. Comparison between conventional indirect competitive enzyme-linked immunosorbent assay (icELISA) and simplified icELISA for small molecules[J]. Anal Chim Acta, 2006, 571(1):79-85.DOI:10.1016/j.aca.2006.04.060.
[12]	郭金博, 施钦, 熊豫武, 等. 盐碱混合胁迫对‘中山杉406’生长及光合特性的影响[J]. 南京林业大学学报(自然科学版), 2019, 62(1):61-68.
	GUO J B, SHI Q, XIONG Y W, et al. Effects of salt-alkaline mixed stress on growth and photosynthetic characteristics of Taxodium hybrid ‘Zhongshanshan 406’[J]. J Nanjing For Univ (Nat Sci Ed), 2019, 62(1):61-68.DOI: 10.3969/j.issn.1000-2006.201805078.
[13]	段娜, 汪季, 郝玉光, 等. 水分变化对荒漠植物白刺气体交换参数及形态特征的影响[J]. 南京林业大学学报(自然科学版), 2019, 43(6):32-38.
	DUAN N, WANG J, HAO Y G, et al. Effects of gas exchange and morphological characteristics of desert species Nitraria tangutorum under moisture variation[J]. J Nanjing For Univ (Nat Sci Ed), 2019, 43(6):32-38.DOI: 10.3969/j.issn.1000-2006.201812036.
[14]	CHOUDHURY B I, KHAN M L, DAYANANDAN S. Functional androdioecy in critically endangered Gymnocladus assamicus (Leguminosae) in the eastern Himalayan region of northeast India[J]. PLoS One, 2014, 9(2):e87287.DOI:10.1371/journal.pone.0087287.
[15]	TEDDER A, HELLING M, PANNELL J R, et al. Female sterility associated with increased clonal propagation suggests a unique combination of androdioecy and asexual reproduction in populations of Cardamine amara (Brassicaceae)[J]. Ann Bot, 2015, 115(5):763-776.DOI:10.1093/aob/mcv006.
[16]	王巧妹, 王继武, 管志勇, 等. 茶用菊七月白×苏菊7号F1代群体与产量相关性状的变异分析及高产植株筛选[J]. 江苏农业学报, 2022, 38(2):512-520.
	WANG Q M, WANG J W, GUAN Z Y, et al. Genetic variation of yield-related traits and screening of high-yield hybrid plants in tea chrysanthemum ‘Qiyuebai’× ‘Suju 7’ F1 segregating progeny[J]. Jiangsu J Agric Sci, 2022, 38(2):512-520.DOI:10.3969/j.issn.1000-4440.2022.02.027.
[17]	王丽梅, 余龙江, 吴耿, 等. 桂花不同花期蒸腾作用和光合作用日变化研究[J]. 现代生物医学进展, 2008, 8(8):1504-1507,1474.
	WANG L M, YU L J, WU G, et al. The transpiration rate and net photosynthesis changes in the flowering stages of Osmanthus fragrans[J]. Prog Mod Biomed, 2008, 8(8):1504-1507,1474.DOI:10.13241/j.cnki.pmb.2008.08.004.
[18]	张建新, 马英杰, 何江勇. 不同施氮量滴灌棉田棉花蕾期脱落酸(ABA)与气孔导度之间的响应关系[J]. 干旱区研究, 2016, 33(3):534-539.
	ZHANG J X, MA Y J, HE J Y. Response relationship between ABA content and stomatal conductance of cotton plant at bud stage under nitrogen application and drip irrigation[J]. Arid Zone Res, 2016, 33(3):534-539.DOI:10.13866/j.azr.2016.03.12.
[19]	房丽莎, 徐自恒, 刘震, 等. 山桐子果实发育过程中内含物、内源激素及光合特性的变化[J]. 林业科学, 2020, 56(11):41-52.
	FANG L S, XU Z H, LIU Z, et al. Changes of contents,endogenous hormones and photosynthetic characteristics of Idesia polycarpa fruit at the different developmental stages[J]. Sci Silvae Sin, 2020, 56(11):41-52.DOI: 10.11707/j.1001-7488.20201105.
[20]	崔院院, 郭先锋, 邢树堂, 等. 脱落酸在牡丹切花衰老中的作用[J]. 中国农学通报, 2015, 31(22):136-141.
	CUI Y Y, GUO X F, XING S T, et al. Role of abscisic acid in senescence of tree peony cut flower[J]. Chin Agric Sci Bull, 2015, 31(22):136-141.DOI: 10.11924/j.issn.1000-6850.casb15010199.
[21]	王玮. 芍药切花衰老过程中ABA、H₂O₂和乙烯的生理作用研究[D]. 洛阳: 河南科技大学, 2013.
	WANG W. Study on the physiological role of ABA,H₂O₂ and ethylene during the aging process of peony cut flower[D]. Luoyang: Henan University of Science and Technology, 2013.
[22]	季彤彤. 茉莉酸诱导H₂O₂的积累调控拟南芥衰老的机理研究[D]. 武汉: 武汉大学, 2020.
	JI T T. Jasmonic acid regulates senescence by inducing H₂O₂ accumulation in Arabidopsis[D]. Wuhan: Wuhan University, 2020.
[23]	ZHANG Y, JI T T, LI T T, et al. Jasmonic acid promotes leaf senescence through MYC₂-mediated repression of CATALASE2 expression in Arabidopsis[J]. Plant Sci, 2020, 299:110604.DOI:10.1016/j.plantsci.2020.110604.
[24]	陈海元, 朱晓妹, 张所兵, 等. 一个水稻斑马叶突变体的遗传分析和基因定位[J]. 江苏农业学报, 2021, 37(1):1-7.
	CHEN H Y, ZHU X M, ZHANG S B, et al. Genetic analysis and gene mapping of a zebra leaf mutant in rice[J]. Jiangsu J Agric Sci, 2021, 37(1):1-7.DOI:10.3969/j.issn.1000-4440.2021.01.001.
[25]	胡绍庆, 宣子灿, 朱诚, 等. 桂花花芽分化期内源激素含量的变化[J]. 西北植物学报, 2011, 31(2):398-400.
	HU S Q, XUAN Z C, ZHU C, et al. Changes of endogenous hormone contents during flower bud differentiation of sweet Osmanthus[J]. Acta Bot Boreali Occidentalia Sin, 2011, 31(2):398-400.DOI:10.3969/j.issn.1000-4025(2011)02-0398-03.

指标 index	花期 flowering stage	G_s	C_i	V_pdl
	初期	0.652^**	-0.369^**	-0.687^**
P_n (雄性male)	盛期	0.660^**	-0.089	0.140
	末期	0.591^**	-0.608^**	-0.322^**
	初期	0.963^**	0.579^**	-0.068
T_r (雄性male)	盛期	0.929^**	0.529^**	-0.014
	末期	0.689^**	0.387^**	0.548^**
	初期	0.485^**	0.185^*	-0.418^**
P_n (两性hermaphroditic)	盛期	0.511^**	-0.289^*	-0.624^**
	末期	0.782^**	0.007	-0.401^**
	初期	0.823^**	0.702^**	0.381^**
T_r (两性hermaphroditic)	盛期	0.955^**	0.749^**	-0.001
	末期	0.978^**	0.558^**	-0.192^*

性别 gender	花期 flowering stage	E_WUE/ (μmol · mmol^-1)	L_S /%	C_E/ (mmol· m^-2·s^-1)
雄性 male	初期	5.156	47.66	0.021
	盛期	4.800	47.28	0.019
	末期	4.327	42.22	0.020
两性 hermaphroditic	初期	7.446	52.90	0.036
	盛期	5.763	50.23	0.028
	末期	3.747	35.94	0.021

雄全异株桂花不同花期光合和内源激素的变化

Photosynthesis and hormone study of male and hermaphroditic Osmanthus fragrans at different flowering stages

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