叶用文冠果总多酚提取工艺及抗氧化活性分析

doi:10.12302/j.issn.1000-2006.202205009

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南京林业大学学报（自然科学版） ›› 2023, Vol. 47 ›› Issue (5): 129-137.doi: 10.12302/j.issn.1000-2006.202205009

叶用文冠果总多酚提取工艺及抗氧化活性分析

苏泾涵¹(), 王改萍¹^,^*(), 刘玉华², 戚亚¹, 彭大庆³, 李守科⁴, 曹福亮¹

1.南京林业大学,南方现代林业协同创新中心,江苏南京 210037
2.江苏农林职业技术学院,江苏句容 212400
3.江苏盐城林场,江苏盐城 224057
4.山东沃奇农业开发有限公司,山东潍坊 262106

收稿日期:2022-05-07 修回日期:2022-09-26 出版日期:2023-09-30 发布日期:2023-10-10
作者简介:苏泾涵(850714113@qq.com)。
基金资助:
江苏省重点研发计划(2020BBA042)

Extraction technology and antioxidant activity analyses of total polyphenols from Xanthoceras sorbifolia leaves

SU Jinghan¹(), WANG Gaiping¹^,^*(), LIU Yuhua², QI Ya¹, PENG Daqing³, LI Shouke⁴, CAO Fuliang¹

1. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
2. Jiangsu Vocational and Technical College of Agriculture and Forestry, Jurong 212400, China
3. Jiangsu Yancheng Forest Farm, Yancheng 224057, China
4. Shandong Woqi Agricultural Development Co., Ltd., Weifang 262106, China

Received:2022-05-07 Revised:2022-09-26 Online:2023-09-30 Published:2023-10-10

摘要/Abstract

摘要：

【目的】探究文冠果(Xanthaceras sorbifolia)叶总多酚最佳提取工艺,比较不同来源文冠果叶总多酚的含量及其抗氧化活性,为叶用文冠果开发利用提供参考。【方法】以15个来源地的文冠果叶为材料,利用超声波辅助法提取文冠果叶总多酚,采用福林酚比色法测定文冠果叶总多酚的含量;通过测定文冠果叶总多酚对DPPH自由基清除能力和抗氧化能力(FRAP法)来评价其综合抗氧化性能。【结果】通过单因素试验,得到文冠果叶总多酚提取率的最适工艺分别为乙醇体积分数70%、料液比1∶30 g/mL、提取时间40 min、提取温度60 ℃。根据Box-Behnken响应面法对文冠果叶总多酚提取率进行优化设计,得到的最佳提取条件为乙醇体积分数60%、料液比1∶30 g/mL、提取时间43 min、提取温度70 ℃。15个来源地文冠果总多酚平均含量为36.34 mg/g,其中宁夏银川文冠果叶总多酚含量最高,为42.37 mg/g。不同来源地文冠果叶总多酚抗氧化能力不同,15个来源地文冠果叶总多酚的DPPH自由基清除率的范围为64.33%~92.65%,银川文冠果叶的DPPH自由基清除率最高,为92.65%;15个来源地文冠果叶总多酚的总抗氧化能力为0.71~1.53 mol/g,青海乐都的总抗氧化能力最强,为1.53 mol/g;乐都的综合抗氧化能力(APC)指数最高,为99.73%;新疆昌吉的APC指数最低,仅为62.86%;文冠果叶总多酚含量与其提取物抗氧化能力整体呈正相关关系。【结论】文冠果叶总多酚最佳提取条件为乙醇体积分数60%、料液比1∶30 g/mL、提取时间43 min、提取温度70 ℃;银川和乐都文冠果叶片总多酚含量较高且抗氧化能力较强。

关键词: 文冠果, 总多酚, 抗氧化活性, 响应面, 超声波

Abstract:

【Objective】This research aims to explore the best extraction process of total polyphenols from Xanthoceras sorbifolia leaves, compare the content and antioxidant activity of total polyphenols from different sources, and provide a reference for the development and utilization of X. sorbifolia leaves. 【Method】The total polyphenols of X. sorbifolia leaves from 15 sources were extracted by ultrasonic assisted response surface method, and the content of total polyphenols in X. sorbifolia leaves was determined by Folin-iocaileu colorimetry. The antioxidant capacity of X. sorbifolia leaves was evaluated by measuring the scavenging power of total polyphenols on the DPPH free radical and the antioxidant capacity of FRAP method. 【Result】Through single factor experiments, the optimum extraction process of total polyphenols from X. sorbifolia leaves was obtained with ethanol concentration 70%, solid-liquid ratio 1∶30 g/mL and extraction temperature 60 ℃. The extraction rate of total polyphenols from X. sorbifolia leaves was optimized according to Box-Behnken response surface method. The optimum extraction conditions were as follows: ethanol concentration 60%, solid-liquid ratio 1∶30 g/mL, extraction time 43 min and extraction temperature 70 ℃. The average content of total polyphenols in 15 sources was 36.34 mg/g, of which the content of total polyphenols in X. sorbifolia leaves in Yinchuan, Ningxia was the highest, 42.37 mg/g. The antioxidant capacity of total polyphenols in X. sorbifolia leaves from different sources was different. The DPPH free radical scavenging rate of total polyphenols in X. sorbifolia leaves from 15 sources ranged from 64.33% to 92.65%, and the DPPH free radical scavenging rate of Yinchuan provenance in Ningxia was the highest, 92.65%. The total antioxidant capacity of total polyphenols in X. sorbifolia leaves from 15 sources ranged from 0.71 to 1.53 mol/g, and the total antioxidant capacity from Ledu provenance in Qinghai was the strongest, 1.53 mol/g. The APC index of Ledu is the highest, 99.73%; Xinjiang Changji has the lowest APC index, only 62.86%.The content of total polyphenols in X. sorbifolia leaves was positively correlated with the antioxidant capacity of its extract. 【Conclusion】The optimum extraction conditions of total polyphenols from X. sorbifolia leaves were ethanol concentration 60%, solid-liquid ratio 1∶30 g/mL, extraction time 43 min and extraction temperature 70 ℃. The total polyphenol contents of X. sorbifolia leaves from Yinchuan in Ningxia and Ledu in Qinghai are high and the antioxidant capacity are strong.

Key words: Xanthoceras sorbifolia, total polyphenols, antioxidant activity, response surface, ultrasonic

中图分类号:

苏泾涵,王改萍,刘玉华,等. 叶用文冠果总多酚提取工艺及抗氧化活性分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 129-137.

SU Jinghan, WANG Gaiping, LIU Yuhua, QI Ya, PENG Daqing, LI Shouke, CAO Fuliang. Extraction technology and antioxidant activity analyses of total polyphenols from Xanthoceras sorbifolia leaves[J].Journal of Nanjing Forestry University (Natural Science Edition), 2023, 47(5): 129-137.DOI: 10.12302/j.issn.1000-2006.202205009.

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方差来源 source	平方和 sum of squares	自由度 df	均方差 mean square	F	P
回归模型 model	196.360	14	14.030	39.920	<0.000 1^**
乙醇体积分数(A) ethanol volume fraction	19.530	1	19.530	55.590	<0.000 1^**
料液比(B) material liquid ratio	0.145	1	0.145	0.413	0.530 7
提取时间(C) extraction time	12.080	1	12.080	34.380	<0.000 1^**
提取温度(D) extraction temperature	123.070	1	123.070	350.250	<0.000 1^**
AB	0.055	1	0.055	0.157	0.697 8
AC	1.010	1	1.010	2.870	0.112 1
AD	4.860	1	4.860	13.840	0.002 3^**
BC	0.476	1	0.476	1.350	0.263 9
BD	0.416	1	0.416	1.180	0.294 9
CD	0.081	1	0.081	0.231	0.638 1
A²	20.470	1	20.470	58.260	<0.000 1^**
B²	4.520	1	4.520	12.860	0.003 0^**
C²	1.610	1	1.610	4.570	0.050 6
D²	2.080	1	2.080	5.910	0.029 0^*

指标 index	抗氧化能力指数/% antioxidant capacity index
指标 index	Ql	Ny	Lc	Ib	Ic	Lj	Xk	Hq	Iw	Sy	Jc	Af	Gz	Hz	Xc
自由基清除率 free radical scavenging rate	99.46	100.00	98.82	93.87	94.01	93.87	90.22	78.51	86.75	86.53	81.04	78.10	84.00	69.43	77.93
总抗氧化能力 total antioxidant capacity	100.00	90.85	86.27	88.89	73.86	66.01	62.09	70.59	60.78	58.82	64.05	63.40	46.41	59.48	47.71
APC	99.73	95.42	92.55	91.38	83.93	79.94	76.16	74.55	73.77	72.68	72.54	70.75	65.20	64.46	62.82
排序 order	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15

指标 index	来源 origin	多酚含量 polyphenol content	DPPH 清除能力 DPPH removal capacity	总抗氧化能力 total antioxidant capacity
总多酚含量 total polyphenol content	0.622^**	1
DPPH清除能力 DPPH removal capacity	0.763^**	0.825^**	1
总抗氧化能力 total antioxidant capacity	0.526^**	0.510^**	0.619^**	1

叶用文冠果总多酚提取工艺及抗氧化活性分析

Extraction technology and antioxidant activity analyses of total polyphenols from Xanthoceras sorbifolia leaves

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序号 No.	乙醇体积分数/% ethanol volume fraction	料液比/ (g·mL^-1) solid- liquid ratio	提取时间/min extraction time	提取温度/℃ extraction temperature	总多酚含量/ (mg·g^-1) totalpoly- phenol content
1	40	1∶20	30	60	35.81
2	50	1∶20	30	60	37.68
3	60	1∶20	30	60	39.17
4	70	1∶20	30	60	40.27
5	80	1∶20	30	60	34.85
6	90	1∶20	30	60	30.05
7	60	1∶10	30	60	35.52
8	60	1∶15	30	60	38.18
9	60	1∶20	30	60	39.41
10	60	1∶25	30	60	39.60
11	60	1∶30	30	60	42.86
12	60	1∶35	30	60	40.50
13	60	1∶20	20	60	34.51
14	60	1∶20	30	60	38.54
15	60	1∶20	40	60	41.86
16	60	1∶20	50	60	40.61
17	60	1∶20	60	60	44.16
18	60	1∶20	70	60	45.54
19	60	1∶20	30	30	22.32
20	60	1∶20	30	40	27.31
21	60	1∶20	30	50	30.69
22	60	1∶20	30	60	40.24
23	60	1∶20	30	70	38.18
24	60	1∶20	30	80	44.50

序号 No.	乙醇体积分数/% ethanol volume fraction	料液比/ (g·mL^-1) liquid ratio	提取时间/min extraction time	提取温度/℃ extraction temperature	总多酚含量/ (mg·g^-1) total polyphenols content
1	50	1∶20	40	60	39.65
2	70	1∶20	40	60	37.69
3	50	1∶30	40	60	39.82
4	70	1∶30	40	60	37.39
5	60	1∶25	30	50	34.85
6	60	1∶25	50	50	37.92
7	60	1∶25	30	70	41.66
8	60	1∶25	50	70	44.16
9	50	1∶25	40	50	34.97
10	70	1∶25	40	50	34.32
11	50	1∶25	40	70	43.66
12	70	1∶25	40	70	38.60
13	60	1∶20	30	60	39.50
14	60	1∶30	30	60	38.64
15	60	1∶20	50	60	39.54
16	60	1∶30	50	60	40.06
17	50	1∶25	30	60	36.64
18	70	1∶25	30	60	35.04
19	50	1∶25	50	60	40.15
20	70	1∶25	50	60	36.54
21	60	1∶20	40	50	37.54
22	60	1∶30	40	50	37.79
23	60	1∶20	40	70	43.10
24	60	1∶30	40	70	44.64
25	60	1∶25	40	60	38.86
26	60	1∶25	40	60	39.33
27	60	1∶25	40	60	39.33
28	60	1∶25	40	60	39.89
29	60	1∶25	40	60	39.33

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