南京林业大学学报(自然科学版) ›› 1993, Vol. 17 ›› Issue (04): 22-26.doi: 10.3969/j.jssn.1000-2006.1993.04.004

• 研究论文 • 上一篇    下一篇

大袋蛾核型多角体病毒、病状及毒力的生物测定

赵博光   

  1. 南京林业大学林学系
  • 出版日期:1993-12-18 发布日期:1993-08-18

THE NUCLEAR POLYHEDROSIS VIRUS OF CLANIA VARIEGATA, ITS SYMPTOM AND BIOASSAY

Zhao Boguang   

  1. Department of Forestry
  • Online:1993-12-18 Published:1993-08-18

摘要: <正>报道了对大袋蛾核型多角体病毒、病状的观察。林间因感染大袋蛾核型多角体病毒而死亡的幼虫可分为两类:一类为纯病毒感染,另一类为病毒和细菌混合感染。用不同浓度的大袋蛾核型多角体病毒感染大袋蛾卵,得多角体浓度与死亡率的直线回归方程:y=0.78十0.823 x,LC_(50)为1.18×10~5PIB/mL。用10~5,5×10~5,10~6PIB/mL感染卵,得感染时间与死亡率的关系和LT_(50),分别为:y=6.45x-0.36,LT_(50)=6.60d;y=5.11x+0.29,LT_(50)=8.35 d;y=4.93x+0.09,LT_(50)=9.91d。

Abstract: The nuclear polyhedrosis virus of Clania variegata (CvNPV) and the sysmptom of the disease were observed. The dead larvae caused by CvNPV fall into two categories: one is caused only by CvNPV, the other by CvNPV and bacteria. Eggs of C. variegata were contaminated with different concentration of the polyhedral inclusion bodies (PIB) of CvNPV, the regression equation was obtained between concentration and mortality: y=0. 78 + 0. 832x and LC50=l. 18× 105PIB/mL. Eggs were contaminated with 105, 5×105, 106 PIB/mL, then relationships was got between time and mortality, LT50. They were y=6. 54×-0. 36, LT50=6. 60 days; y = 5.11x+0. 29, LT50= 8. 35 days; y= 4. 93x + 0. 09, LT50 = 9. 91 days respectively.