南京林业大学学报(自然科学版) ›› 1996, Vol. 20 ›› Issue (01): 73-76.doi: 10.3969/j.jssn.1000-2006.1996.01.017

• 研究论文 • 上一篇    下一篇

刺槐根瘤菌优良菌株的初步筛选

韩素芬;李光友;吴晋东   

  1. 南京林业大学森林资源与环境学院;南京210037;南京林业大学森林资源与环境学院;南京210037;南京林业大学森林资源与环境学院;南京210037
  • 出版日期:1996-03-18 发布日期:1996-02-18

SCREENING EFFICIENT STRAINS OF LOCUST (ROBINIA PSEUDOACACIA) NODULE BACTERIA

Han sufeng Li guanyou Wu jindong   

  1. College of Forest Resources and Environment Nanjing Forestry University Nanjing 210037
  • Online:1996-03-18 Published:1996-02-18

摘要: <正>从豆科植物根瘤分离获得的89个根瘤菌株,经刺槐水培和盆栽接种试验,92-1-11、84-21、92-125三个菌株,不仅在刺槐根部形成的根瘤多且大,根瘤的乙炔还原固氮酶活性每克鲜瘤温育1h乙炔还原为乙烯量接近或超过了1000μmol,超过了三叶草优良根瘤菌株和高效大豆根瘤菌株的固氮水平,对刺槐生长有明显促进作用,生长二个月的植株平均鲜重超过5g,比对照高出82%以上,故认为这三个菌株为刺槐初筛的优良菌株,可供生产中试用,

Abstract: 89 strains of root nodule bacteria from different species of tree legumes were isolated following the method suggested by Vincent (1970). Inoculation of locust water culture and pot experiments confirmed that strains 92-1-11, 84-21, and 92-125 not only formed nodules on locust root both large and abundance, but show great nitrogenase activity more than 1000 (amolC2H4/g(fresh nodule)·h, and go beyond the level of the nitrogen fixed by efficient soybean root nodule bacteria. They bring about a great advance in locust growth. The average fresh weight of two months plant exceed 5 grams, show a marked increase over CK-plant, more than 82%. We consider that 92-1-11, 84-21 and 92-125 are efficient strains, can accomodate as probationary strains used in forest production.