Abstract: The endogenous GAs (GA1＋3，GA4＋7),ABA and CTKs (iPAs, ZRs, DHZRs,)levels in male and female strobili and its nearby shoot apex of three types (Androdynamous, Gynodynamus and Sex-balanced) in masson pine were estimated with ELISAs. The results showed that there were higher levels of GAs in female strobili, and higher levels of ABA in male strobili, and much lower levels of ZRs in male or female strobili than in its nearby shoot apex. On the other hand, there was no clear difference between the GAs contents of male strobili or the ABA contents of female strobili respectively, in comparison with that in its nearby shoot apex. These indicated that female flowering was associated with higher ratio of GAs/ZRs and male flowering with higher ratio of ABA/ZRs. The experimental results of the chemical regulation indicated that ABA obviously enhanced the formation of male strobili, and GA3，GA4＋7 and NAA significantly promoted the formation of female and male strobili.