摘要
<正>用硫酸铵分级沉淀 ,葡聚糖凝胶分离技术 ,对荧光假单胞菌 (Pseudomonasflu .)所产的半纤维素酶进行分离纯化 ,得到单一组分的木聚糖酶。通过凝胶电泳法 ,测得其摩尔式量为330 0 0。研究表明 ,该酶的最适pH值为 6 0 ,最适温度 60℃。酶的动力学研究测出米氏常数Km 值为 4 76mg/mL
Abstract
Using the systematical process for isolation and purification of xylanase from Pseudomonas flu. with the tehnique (NH) 2SO 4 fractionation and Sephede x G100 gel column, we obtained the xylanase showing homogeneity in the gel electrophorosis.The melecular weight was 33?000 by SDS polyacrylamide.The optimum pH,temprature were 6.0 and 60?℃,respectively.The studies of enzyme kinetics showed K m=4.76?mg/mL.
苏玉萍;王书翰;欧凯.
荧光假单胞菌产木聚糖酶的纯化和性质[J]. 南京林业大学学报（自然科学版）. 2000, 24(02): 60-62 https://doi.org/10.3969/j.jssn.1000-2006.2000.02.018
Su Yuping\ Wang Shuhan\ Ou Kai.
Purification and Properties of Xylanase from Pseudomonas flu.[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2000, 24(02): 60-62 https://doi.org/10.3969/j.jssn.1000-2006.2000.02.018
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