南京林业大学学报(自然科学版) ›› 2002, Vol. 26 ›› Issue (02): 73-76.doi: 10.3969/j.jssn.1000-2006.2002.02.020

• 研究论文 • 上一篇    下一篇

福建山樱花的组织培养及植株再生

王光萍;黄敏仁   

  1. 南京林业大学林木遗传和基因工程重点实验室;江苏南京210037;南京林业大学林木遗传和基因工程重点实验室;江苏南京210037
  • 出版日期:2002-04-18 发布日期:2002-04-18

Tissue Culture and Plant Regeneration of Cerasus campanulata

WANG Guang ping,HUANG Min ren   

  1. The Key Laboratory of Forestry Genetics & Gene Engineering Nanjing Forestry University,Nanjing 210037,China
  • Online:2002-04-18 Published:2002-04-18

摘要: <正>以春季萌发的福建山樱花嫩枝为外植体诱导丛生芽。在 1 /4MS +BA1 .0mg/L +IBA0 .0 1mg/L的培养基上 ,其丛生芽诱导率达 87.5 % ;在继代培养中选择MS +BA1 .0mg/L +IBA0 .1mg/L +GA30 .3mg/L或KT1 .0mg/L +NAA0 .1mg/L +GA30 .3mg/L培养基 ,不定芽增殖较快。赤霉素对其不定芽的伸长有明显效果 ,当芽伸长至 3~ 4cm时 ,切下置于生根培养基 1 /2MS +NAA1 .0mg/L +IBA1 .0mg/L +BA0 .75mg/L中 ,生根率达 90 %以上 ,移栽成活率达 95 %。

Abstract: Stabilized shoot cultures were achieved when the explant sources were the juvenile branches of Cerasus campanulata in spring.The inducing rate of fasciculated shoots was 87.5% when the inducing medium of 1/4MS with BA 1.0 mg/L and IBA 0.01 mg/L were used.The adventitious shoots were propagated most rapidly when subcultured on the medium of MS with BA 1.0 mg/L,IBA 0.1 mg/L and GA 3 0.3 mg/L,or MS with KT1.0 mg/L,NAA 0.1 mg/L and GA 3 0.3 mg/L.The elongation of adventitious shoots was greatly affected by gibberellin.The adventitious shoots were cut and rooted in the rooting medium of 1/2MS with NAA 1.0 mg/L IBA 1.0 mg/L and BA 0.75 mg/L.The tooting rate was more than 90%.The surviving rate was 95% when the intact shoots were moved to the greenhouse.

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