Abstract: Small pieces of young leaves of Dianthus chinensis L.collected from the campus of Nanjing Forestry University in spring were cultured on the differentiation medium of MS+6BA(2.0 mg/L)+2,4D(0.2 mg/L) after sterilization.Green yellow calli were induced from the explants after one week of culture and then were subcultured on the differentiation medium of MS+KT(0.3 mg/L)+6BA(0.3 mg/L)+NAA(0.1 mg/L).Four weeks later,shoots appeared in some culture bottles.4～5 cm long shoots were cut at the bottom and the same amount of shoots(60) were cultured on control MS medium and treat MS media supplemented with 0.05 mmol/L putresine(Put),spermidine(Spd),spermine(Spm)and 5 mmol/L difluoromethylornithine(DFMO),difluoromethylarginine(DFMA),cyclohexyl ammolonium sulphat(CHAS)respectively.The experiments were repeated 4 times.Flower buds appeared on some media two weeks after inoculation and then flowered 4～5 days later.The apexes were collected every 5 days from the beginning day of culture for detecting the contents of zeatin tiboside group (ZRs),isopentenyl adenine group(iPAs) and indole3acetic acid(IAA).The culture results showed that Spd and Spm promoted flowering significantly,and the flowering rate was raised from the control of 38.3% to 48.3% and 60.0% respectively.Put and DFMA didn’t affect flowering much while DFMO and CHAS inhibited flowering totally.Endogenous hormone content detection results showed that Spd and Spm enhanced the contents of ZRs,iPAs and IAA while DFMO decreased them notably,and Put didn’t affect them much.CHAS and DFMA enhanced the contents of ZRs and iPAs while decreasing the contents of IAA,and the effects of DFMA were not as notable as that of CHAS.