Abstract: After -6 ℃ natural low temperature disaster,the physiological indices of cold resistance in 1 year seedlings of three kinds of Michelia with three species were detected.The results demonstrated that the species with high content of soluble sugar appeared strong cold resistance.The content of MDA increases with its cold resistance decreasing within certain range and while freezing seriously,its content decreases.There is no remarkable relationship between the content of proline and cold resistance.According to these indices and results from field,we discovered that the alignment of cold resistance of Michelia was M.maudiae>M.chapensis>M.macclurei.The alignment of provenance in M.maudiae of that was Hangzhou>Huangshan;The cold resistance of provenance in M.chapensi was Hangzhou slightly higher than Changsha.The indices of soluble sugar and MDA can be as cold resistance index of Michelia,and may be used to select cold resistance.The content of proline only can be as a reference index.